Chol-N3 

Chol-N3 is a bioorthogonal-based chol probe. Chol-N3 can combine with super-resolution fluorescence microscopy (SRM), providing direct visualization of nanoscale lipid heterogeneity in the cell surface of resting living cells^[1]. Chol-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Chol-N3 (25 μM; 16 h; living SH-SY5Y cells) distribution in living cells clearly shows the presence of nanoscopic domains in the PM^[1].
Chol-N3 (15 μM; 16 h; living SH-SY5Y cells) has nanoscale spatiotemporal diffusion dynamics in SH-SY5Y cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

385.59

C₂₄H₃₉N₃O

2106868-12-4

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCN=[N+]=[N-])([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4(C(C[C@H](CC4)O)=CC3)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lorizate M, et, al. Super-Resolution Microscopy Using a Bioorthogonal-Based Cholesterol Probe Provides Unprecedented Capabilities for Imaging Nanoscale Lipid Heterogeneity in Living Cells. Small Methods. 2021 Sep;5(9):e2100430.  [Content Brief]