2. PI3K/Akt/mTOR
  3. PI3K Akt
  4. Cholesta-3,5-diene

Cholesta-3,5-diene  (Synonyms: delta-3,5-Cholestadiene)

Cat. No.: HY-W127575 Purity: 99.54%
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Cholesta-3,5-diene is an inflammatory modulator that targets immune cells such as neutrophils and accelerates wound healing by promoting neutrophil chemotaxis and fibroblast migration. Cholesta-3,5-diene enhances immune cell recruitment and extracellular matrix deposition by activating chemokine receptor-mediated signaling pathways such as PI3K/Akt. Cholesta-3,5-diene can be applied topically to wound repair and has potential therapeutic value in chronic ulcers or skin lesions.

For research use only. We do not sell to patients.

Cholesta-3,5-diene Chemical Structure

Cholesta-3,5-diene Chemical Structure

CAS No. : 747-90-0

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Based on 1 publication(s) in Google Scholar

Cholesta-3,5-diene Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Cholesta-3,5-diene is an inflammatory modulator that targets immune cells such as neutrophils and accelerates wound healing by promoting neutrophil chemotaxis and fibroblast migration. Cholesta-3,5-diene enhances immune cell recruitment and extracellular matrix deposition by activating chemokine receptor-mediated signaling pathways such as PI3K/Akt. Cholesta-3,5-diene can be applied topically to wound repair and has potential therapeutic value in chronic ulcers or skin lesions[1][2].

In Vivo

Neutrophil Recruitment: Cholesta-3,5-diene (30 μg/ml; topical application; single dose) promotes neutrophil recruitment to tailfin wounds in transgenic zebrafish larvae (Tg(mpx:GFP)) by 6 h post-treatment1.
Tissue Regeneration: Cholesta-3,5-diene (30 μg/ml; topical application; single dose) does not enhance tissue regeneration in zebrafish tailfin wounds, unlike the lipid fraction Ft containing Cholesta-3,5-diene1.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Transgenic zebrafish larvae (Tg(mpx:GFP), 4 days post-fertilization, male/female mixed, 200-300 mg)[1]
Dosage: 30 µg/mL Cholesta-3,5-diene (dissolved in ethanol, diluted in E2 medium)
Administration: Topical application to transected tailfin immediately after wounding, single dose
Result: Significantly increased neutrophil accumulation at the wound site by 6 h post-treatment (2-fold vs. vehicle control.
No effect was observed in uninjured tailfins[1].
Animal Model: Transgenic zebrafish larvae (Tg(mpx:GFP), 4 days post-fertilization, male/female mixed, 200-300 mg)1
Dosage: 30 µg/mL Cholesta-3,5-diene (dissolved in ethanol, diluted in E2 medium)
Administration: Topical application to transected tailfin immediately after wounding, single dose
Result: Did not promote significant tissue regeneration at 2 or 6 days post-treatment, unlike the lipid fraction Ft (which showed dose-dependent regeneration).
Resulted maximum regeneration with Ft of 50 µg/mL.
Molecular Weight

368.64

Formula

C27H44
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C=CCC[C@]4(C)[C@@]3([H])CC[C@]12C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 2 mg/mL (5.43 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7127 mL 13.5634 mL 27.1267 mL
5 mM 0.5425 mL 2.7127 mL 5.4253 mL
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* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.54%

SDS (392 KB)

COA (250 KB) HNMR (251 KB) RP-HPLC (262 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.7127 mL 13.5634 mL 27.1267 mL 67.8168 mL
5 mM 0.5425 mL 2.7127 mL 5.4253 mL 13.5634 mL
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Cholesta-3,5-diene Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cholesta-3,5-diene747-90-0delta-3,5-CholestadienePI3KAktPhosphoinositide 3-kinasePKBProtein kinase Bbiochemical assayreagentInhibitorinhibitorinhibit

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