2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. CM-H2DCFDA

CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive.

For research use only. We do not sell to patients.

CM-H2DCFDA Chemical Structure

CM-H2DCFDA Chemical Structure

CAS No. : 850013-49-9

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50 μg USD 180 In-stock

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Based on 3 publication(s) in Google Scholar

CM-H2DCFDA Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive[1].

In Vitro

Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). ROS Measurement: 1. Incubate the cells according to your normal protocol. 2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness. 3. Wash the excess probe. 4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CM-H2DCFDA Related Antibodies
Molecular Weight

577.79

Formula

C27H19Cl3O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(OC1=C(Cl)C=C2C(C3=C(C=CC(CCl)=C3)C(OC(C)=O)=O)C4=CC(Cl)=C(OC(C)=O)C=C4OC2=C1)=O.CC(OC5=C(Cl)C=C6C(C7=C(C=C(CCl)C=C7)C(OC(C)=O)=O)C8=CC(Cl)=C(OC(C)=O)C=C8OC6=C5)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 3 mg/mL (5.19 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7307 mL 8.6537 mL 17.3073 mL
5 mM 0.3461 mL 1.7307 mL 3.4615 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥92.0%

COA (247 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7307 mL 8.6537 mL 17.3073 mL 43.2683 mL
5 mM 0.3461 mL 1.7307 mL 3.4615 mL 8.6537 mL
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CM-H2DCFDA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CM-H2DCFDA850013-49-9Reactive Oxygen Specieslight-sensitivecellular oxidant levelsROSInhibitorinhibitorinhibit

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