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  3. Biochemical Assay Reagents Calcium Channel
  4. Coelenterazine h hydrochloride

Coelenterazine h hydrochloride  (Synonyms: 2-Deoxycoelenterazine hydrochloride; CLZN-h hydrochloride)

Cat. No.: HY-D1024A Purity: 98.80%
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Coelenterazine h (2-Deoxycoelenterazine) hydrochloride, a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h hydrochloride is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations.

For research use only. We do not sell to patients.

Coelenterazine h hydrochloride Chemical Structure

Coelenterazine h hydrochloride Chemical Structure

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5 mg USD 280 In-stock
10 mg USD 450 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of Coelenterazine h hydrochloride:

Coelenterazine h hydrochloride Related Antibodies

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2 Publications Citing Use of MCE Coelenterazine h hydrochloride

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Description

Coelenterazine h (2-Deoxycoelenterazine) hydrochloride, a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h hydrochloride is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations[1][2][3][4].

In Vitro

Coelenterazine h (1-10 μM) hydrochloride can be used as the luminescent substrate for RLuc8[4].
In the measurements of Ca2+ binding kinetics of BRAC, emission intensity of Venus (530 nm) from BRAC are monitored at 1 kHz just after rapid mixing of 5 nM BRAC protein with 20 μM coelenterazine-h hydrochloride in various concentration of Ca2+ buffer[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coelenterazine h hydrochloride Related Antibodies
Molecular Weight

443.92

Formula

C26H22ClN3O2
Appearance

Solid

Color

Light yellow to orange

SMILES

OC(C=C1)=CC=C1C2=CN(C(C(CC3=CC=CC=C3)=N4)=O)C4=C(N2)CC5=CC=CC=C5.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (225.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2527 mL 11.2633 mL 22.5266 mL
5 mM 0.4505 mL 2.2527 mL 4.5053 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2527 mL 11.2633 mL 22.5266 mL 56.3165 mL
5 mM 0.4505 mL 2.2527 mL 4.5053 mL 11.2633 mL
10 mM 0.2253 mL 1.1263 mL 2.2527 mL 5.6316 mL
15 mM 0.1502 mL 0.7509 mL 1.5018 mL 3.7544 mL
20 mM 0.1126 mL 0.5632 mL 1.1263 mL 2.8158 mL
25 mM 0.0901 mL 0.4505 mL 0.9011 mL 2.2527 mL
30 mM 0.0751 mL 0.3754 mL 0.7509 mL 1.8772 mL
40 mM 0.0563 mL 0.2816 mL 0.5632 mL 1.4079 mL
50 mM 0.0451 mL 0.2253 mL 0.4505 mL 1.1263 mL
60 mM 0.0375 mL 0.1877 mL 0.3754 mL 0.9386 mL
80 mM 0.0282 mL 0.1408 mL 0.2816 mL 0.7040 mL
100 mM 0.0225 mL 0.1126 mL 0.2253 mL 0.5632 mL
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Coelenterazine h hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coelenterazine h2-DeoxycoelenterazineCLZN-hBiochemical Assay ReagentsCalcium ChannelCa2+ channelsCa channelsLuminescent SubstratecalciumInhibitorinhibitorinhibit

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