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  4. Copper Fluor-4

Copper Fluor-4  (Synonyms: CF4)

Cat. No.: HY-150086 Purity: 98.44%
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Copper Fluor-4 (CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 has high copper selectivity with a Kd value of 2.9×10−13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).

For research use only. We do not sell to patients.

Copper Fluor-4 Chemical Structure

Copper Fluor-4 Chemical Structure

CAS No. : 2365532-64-3

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5 mg USD 500 In-stock
10 mg USD 800 In-stock
25 mg USD 1600 In-stock
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Based on 1 publication(s) in Google Scholar

Copper Fluor-4 Related Antibodies

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Description

Copper Fluor-4 (CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 has high copper selectivity with a Kd value of 2.9×10−13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission)[1][2][3].

In Vitro

Copper Fluor-4 (0.8 µM, 10 min) demonstrates localization of the copper in the soma region, which includes its localization in the perinuclear Golgi region in the PC-12-derived neurons[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Copper Fluor-4 Related Antibodies
In Vivo

Copper Fluor-4 (2 µM, 24 h) can be used to assess changes in labile copper distributions in small living organisms such as zebrafish embryos[1].
Copper Fluor-4 (10 µM, 3 h) can be used to assess labile Cu levels with a Kd value of 2.9×10−13 M for a 1:1 copper:probe stoichiometry that is well-matched to monitor labile Cu pools by reversible Cu binding without depleting the total Cu store in worms[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

749.05

Formula

C38H47F3N2O2S4
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C1C=CC2=C(C3=C(C(F)(F)F)C=C(CN(CCSCCSCC)CCSCCSCC)C=C3)C4=C(C=C(N5CCCCC5)C=C4)OC2=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (166.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3350 mL 6.6751 mL 13.3502 mL
5 mM 0.2670 mL 1.3350 mL 2.6700 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3350 mL 6.6751 mL 13.3502 mL 33.3756 mL
5 mM 0.2670 mL 1.3350 mL 2.6700 mL 6.6751 mL
10 mM 0.1335 mL 0.6675 mL 1.3350 mL 3.3376 mL
15 mM 0.0890 mL 0.4450 mL 0.8900 mL 2.2250 mL
20 mM 0.0668 mL 0.3338 mL 0.6675 mL 1.6688 mL
25 mM 0.0534 mL 0.2670 mL 0.5340 mL 1.3350 mL
30 mM 0.0445 mL 0.2225 mL 0.4450 mL 1.1125 mL
40 mM 0.0334 mL 0.1669 mL 0.3338 mL 0.8344 mL
50 mM 0.0267 mL 0.1335 mL 0.2670 mL 0.6675 mL
60 mM 0.0223 mL 0.1113 mL 0.2225 mL 0.5563 mL
80 mM 0.0167 mL 0.0834 mL 0.1669 mL 0.4172 mL
100 mM 0.0134 mL 0.0668 mL 0.1335 mL 0.3338 mL
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Copper Fluor-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Copper Fluor-42365532-64-3CF4Copper Fluor4Copper Fluor 4CF 4CF-4Fluorescent DyeCopperCu+ probeInhibitorinhibitorinhibit

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