Coppersensor 1 

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu⁺ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu⁺) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1][2].
A. Preparation of reagent stock solutions for imaging:
1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.
2. Prepare a 10 mM stock solution of CuCl or CuCl₂.
B. CS1 labeling of live cells:
1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.
2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.
3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer.
4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.
(Higher concentrations of dye may result in high levels of background fluorescence)
5. Incubate for 5-20 min in the dark at 25 or 37℃.
C. Imaging the CS1-labeled cells:
1. Imageing in standard confocal microscope with 543 nm excitation.
(CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

629.81

C₃₀H₅₀BF₂N₃S₄

874748-20-6

Solid

Brown to red

576

543

[F-][B+3]1([N]2=C(C)C(CC)=C(C)C2=C(CN(CCSCCSCC)CCSCCSCC)C3=C(C)C(CC)=C(C)[N-]31)[F-]

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Evan W Miller, et al. Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nat Protoc. 2006;1(2):824-7.  [Content Brief]

  [2]. Li Zeng, et al. A selective turn-on fluorescent sensor for imaging copper in living cells. J Am Chem Soc. 2006 Jan 11;128(1):10-1.  [Content Brief]