2. Membrane Transporter/Ion Channel Neuronal Signaling Metabolic Enzyme/Protease
  3. iGluR Endogenous Metabolite
  4. Crocetin

Crocetin  (Synonyms: Transcrocetin; trans-Crocetin)

Cat. No.: HY-N2072 Purity: 98.22%
SDS COA Handling Instructions Technical Support

Crocetin (Transcrocetin), extracted from saffron (Crocus sativus L.), acts as an NMDA receptor antagonist with high affinity. Crocetin is capable of crossing the blood-brain barrier and reach the central nervous system (CNS).

For research use only. We do not sell to patients.

Crocetin Chemical Structure

Crocetin Chemical Structure

CAS No. : 27876-94-4

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Based on 1 publication(s) in Google Scholar

Other Forms of Crocetin:

Crocetin Related Antibodies

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AMPA Receptor NMDA Receptor Kainate Receptor

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Crocetin (Transcrocetin), extracted from saffron (Crocus sativus L.), acts as an NMDA receptor antagonist with high affinity[1]. Crocetin is capable of crossing the blood-brain barrier and reach the central nervous system (CNS)[2].

IC50 & Target

NMDA Receptor

 

Cellular Effect
Cell Line Type Value Description References
RAW264.7 IC50
5.99 μM
Compound: 18
Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method
Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method
[PMID: 23305920]
In Vitro

Crocetin, a saffron metabolite originating from the crocin apocarotenoids, has been shown to exert strong NMDA receptor affinity and is thought to be responsible for the CNS activity of saffron.To ensure unchanged viability of Caco-2 cells throughout the transport experiments, cellular mitochondrial dehydrogenase activity of Caco-2 cells is measured by MTT assay after a 24 h incubation period with the test compounds: Hydroalcoholic saffron extract saffron extract (SE, 0.5-1 mg/mL) and crocin-1 (250-1000 μM) reveal no negative significant changes in cellular viability. Crocetin at 10 μM level does not change viability while higher concentrations (40-160 μM) reduces significantly cellular viability[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Crocetin Related Antibodies
Molecular Weight

328.40

Formula

C20H24O4
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(O)/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (60.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0451 mL 15.2253 mL 30.4507 mL
5 mM 0.6090 mL 3.0451 mL 6.0901 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[1]

Cytotoxicity of test compounds is determined by MTT assay using Caco-2 cells in 96 well plates at a density of 20.000 cells per well in 200 µl FBS-free medium, grown for 96 h and followed by 24 h contact time with the test compounds (100 µL of serum-free media containing SE 0.5, 1, and 2 mg/mL; trans-crocin-1 250, 500, and 1000 µM; Transcrocetin 10, 40, 80, and 160 µM) and incubation at 37°C/5% CO2. The incubation solutions are aspirated, each well is washed twice with 150 µL of PBS and 50 µL of MTT solution are added (2.5 mg/mL in PBS). Supernatants are discarded and the formed formazan is dissolved in 50 µL of DMSO. The absorption of the resulting solution is determined at λ=492 nm against reference wavelength λ=690 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0451 mL 15.2253 mL 30.4507 mL 76.1267 mL
5 mM 0.6090 mL 3.0451 mL 6.0901 mL 15.2253 mL
10 mM 0.3045 mL 1.5225 mL 3.0451 mL 7.6127 mL
15 mM 0.2030 mL 1.0150 mL 2.0300 mL 5.0751 mL
20 mM 0.1523 mL 0.7613 mL 1.5225 mL 3.8063 mL
25 mM 0.1218 mL 0.6090 mL 1.2180 mL 3.0451 mL
30 mM 0.1015 mL 0.5075 mL 1.0150 mL 2.5376 mL
40 mM 0.0761 mL 0.3806 mL 0.7613 mL 1.9032 mL
50 mM 0.0609 mL 0.3045 mL 0.6090 mL 1.5225 mL
60 mM 0.0508 mL 0.2538 mL 0.5075 mL 1.2688 mL
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Crocetin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Crocetin27876-94-4Transcrocetin trans-CrocetiniGluREndogenous MetaboliteIonotropic glutamate receptorsInhibitorinhibitorinhibit

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