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  3. Cy3 maleimide chloride

Cy3 maleimide chloride is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) containing maleimide functional groups. Cy3 is a fluorescent dye with a fluorescence spectrum typically in the green to orange wavelength range. The alkyne functional group of Cy3 maleimide chloride can undergo a "thiol-acrylamide" reaction with molecules containing sulfur-oxygen functional groups to form covalent bonds. Cy3 maleimide chloride can bind to biological molecules such as proteins and antibodies to track their location and dynamic changes in biological samples.

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Cy3 maleimide chloride Chemical Structure

Cy3 maleimide chloride Chemical Structure

CAS No. : 1838643-41-6

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Description

Cy3 maleimide chloride is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) containing maleimide functional groups. Cy3 is a fluorescent dye with a fluorescence spectrum typically in the green to orange wavelength range. The alkyne functional group of Cy3 maleimide chloride can undergo a "thiol-acrylamide" reaction with molecules containing sulfur-oxygen functional groups to form covalent bonds. Cy3 maleimide chloride can bind to biological molecules such as proteins and antibodies to track their location and dynamic changes in biological samples.

Molecular Weight

615.20

Formula

C36H43ClN4O3

CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C1C=CC(N1CCNC(CCCCCN2C3=C(C(C)(C)/C2=C\C=C\C4=[N+](C)C5=C(C4(C)C)C=CC=C5)C=CC=C3)=O)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (203.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6255 mL 8.1274 mL 16.2549 mL
5 mM 0.3251 mL 1.6255 mL 3.2510 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6255 mL 8.1274 mL 16.2549 mL 40.6372 mL
5 mM 0.3251 mL 1.6255 mL 3.2510 mL 8.1274 mL
10 mM 0.1625 mL 0.8127 mL 1.6255 mL 4.0637 mL
15 mM 0.1084 mL 0.5418 mL 1.0837 mL 2.7091 mL
20 mM 0.0813 mL 0.4064 mL 0.8127 mL 2.0319 mL
25 mM 0.0650 mL 0.3251 mL 0.6502 mL 1.6255 mL
30 mM 0.0542 mL 0.2709 mL 0.5418 mL 1.3546 mL
40 mM 0.0406 mL 0.2032 mL 0.4064 mL 1.0159 mL
50 mM 0.0325 mL 0.1625 mL 0.3251 mL 0.8127 mL
60 mM 0.0271 mL 0.1355 mL 0.2709 mL 0.6773 mL
80 mM 0.0203 mL 0.1016 mL 0.2032 mL 0.5080 mL
100 mM 0.0163 mL 0.0813 mL 0.1625 mL 0.4064 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cy3 maleimide chloride
Cat. No.:
HY-D1871
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