2. Metabolic Enzyme/Protease
  3. Oxidative Phosphorylation Mitochondrial Metabolism
  4. Cyanine5 alkyne

Cyanine5 alkyne  (Synonyms: Alkyne-Cy5)

Cat. No.: HY-137042 Purity: 98.15%
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Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC). Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

Cyanine5 alkyne Chemical Structure

Cyanine5 alkyne Chemical Structure

CAS No. : 1223357-57-0

Size Price Stock Quantity
5 mg USD 178 In-stock
10 mg USD 268 In-stock
25 mg USD 510 In-stock
50 mg USD 770 In-stock
100 mg USD 1155 In-stock
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Based on 1 publication(s) in Google Scholar

Cyanine5 alkyne Related Antibodies

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Description

Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC)[1][2]. Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

In Vitro

Cyanine5 alkyne (Alkyne-Cy5) (1-1000 nM) significantly inhibits 3D mammosphere formation in MCF7 cells between 500 nM and 1000 nM and can act as a mitochondrial oxidative phosphorylation (OXPHOS) inhibitor, inducing glycolysis to compensate for mitochondrial ATP depletion[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyanine5 alkyne Related Antibodies
Molecular Weight

556.18

Formula

C35H42ClN3O
CAS No.
Appearance

Solid

Color

Brown to black

Emission (Em)

670
Excitation (Ex)

645
SMILES

C#CCNC(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)C(C)(C)C4=C1C=CC=C4)=O.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (224.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7980 mL 8.9899 mL 17.9798 mL
5 mM 0.3596 mL 1.7980 mL 3.5960 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7980 mL 8.9899 mL 17.9798 mL 44.9495 mL
5 mM 0.3596 mL 1.7980 mL 3.5960 mL 8.9899 mL
10 mM 0.1798 mL 0.8990 mL 1.7980 mL 4.4949 mL
15 mM 0.1199 mL 0.5993 mL 1.1987 mL 2.9966 mL
20 mM 0.0899 mL 0.4495 mL 0.8990 mL 2.2475 mL
25 mM 0.0719 mL 0.3596 mL 0.7192 mL 1.7980 mL
30 mM 0.0599 mL 0.2997 mL 0.5993 mL 1.4983 mL
40 mM 0.0449 mL 0.2247 mL 0.4495 mL 1.1237 mL
50 mM 0.0360 mL 0.1798 mL 0.3596 mL 0.8990 mL
60 mM 0.0300 mL 0.1498 mL 0.2997 mL 0.7492 mL
80 mM 0.0225 mL 0.1124 mL 0.2247 mL 0.5619 mL
100 mM 0.0180 mL 0.0899 mL 0.1798 mL 0.4495 mL
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Cyanine5 alkyne Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyanine5 alkyne1223357-57-0Alkyne-Cy5Oxidative PhosphorylationMitochondrial Metabolismfluorescent dyeazidepost-translational modificationsglycosylationmitochondrial OXPHOSanti-cancerInhibitorinhibitorinhibit

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