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  4. DAPI dilactate

DAPI dilactate  (Synonyms: 4',6-Diamidino-2-phenylindole dilactate)

Cat. No.: HY-D1738 Purity: 99%
SDS COA Handling Instructions Technical Support

DAPI (dilactate) is a blue fluorescent dye that preferentially binds dsDNA and binds to minor groove AT clusters. DAPI (dilactate) is combined with dsDNA, and the fluorescence was enhanced about 20-fold. DAPI (dilactate) can be used to identify the cell cycle and specifically stains the nucleus but not the cytoplasm. DAPI (dilactate) form is more soluble in water than DAPI (dihydrochloride) form.

For research use only. We do not sell to patients.

DAPI dilactate Chemical Structure

DAPI dilactate Chemical Structure

CAS No. : 28718-91-4

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Customer Review

Based on 64 publication(s) in Google Scholar

Other Forms of DAPI dilactate:

DAPI dilactate Related Antibodies

Top Publications Citing Use of Products

59 Publications Citing Use of MCE DAPI dilactate

IF

    DAPI dilactate purchased from MedChemExpress. Usage Cited in: Pharmaceuticals. 2022 Jun 6;15(6):721.

    The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

    DAPI dilactate purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123.  [Abstract]

    Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

    DAPI dilactate purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952.  [Abstract]

    Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

    DAPI dilactate purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15;96:468-479.  [Abstract]

    CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

    DAPI dilactate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146.  [Abstract]

    MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.

    • Biological Activity

    • Purity & Documentation

    • References

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    Description

    DAPI (dilactate) is a blue fluorescent dye that preferentially binds dsDNA and binds to minor groove AT clusters. DAPI (dilactate) is combined with dsDNA, and the fluorescence was enhanced about 20-fold. DAPI (dilactate) can be used to identify the cell cycle and specifically stains the nucleus but not the cytoplasm. DAPI (dilactate) form is more soluble in water than DAPI (dihydrochloride) form. [1][2]

    In Vitro

    General protocol
    1. Preparation of DAPI dilactate working solution
    1.1 Preparation of stock solution
    Dissolve 5 mg DAPI dilactate in 1 mL ddH2O to obtain 5 mg /mL stock solution (DAPI, dilactate 10.9 mM).
    Note: It is recommended to store the stock solution at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
    1.2 Preparation of DAPI dilactate working solution
    The stock solution was diluted 1:5000 in PBS to 1 μg/mL. Store the working solution at 4 °C.
    Note: Please adjust the concentration of DAPI dilactate working solution as needed
    2. Cell staining
    2.1 Suspension cells (96-well plate)
    a. Centrifuge at 1000 g for 3-5 min at 4°C and discard the supernatant. Wash with PBS twice, 5 minutes each time. The cell density is 1×106/mL.
    b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes.
    c. Centrifuge at 400 g for 3-4 minutes at 4°C and discard the supernatant.
    d. Wash 2 times with PBS, 5 min each.
    e. Resuspend cells in serum-free cell culture medium or PBS. Fluorescence microscopy or flow cytometry observation.
    2.2 Adherent cells
    a. Adherent cells were cultured on sterile coverslips.
    b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, shake gently to completely cover the cells, and incubate at room temperature for 3-10 minutes.
    d. Wash 2 times with medium, 5 min each. Fluorescence microscope or flow cytometry observation.
    Precautions
    For long-term storage the stock solution can be aliquoted and stored at ≤–20°C. For shortterm storage the solution can be kept at 2–6°C, protected from light. When handled properly, DAPI solutions are stable for at least six months.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DAPI dilactate Related Antibodies
    Molecular Weight

    457.48

    Formula

    C22H27N5O6
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C(C)O)O.N=C(C1=CC=C(C2=CC3=CC=C(C(N)=N)C=C3N2)C=C1)N.O=C(C(C)O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (218.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1859 mL 10.9294 mL 21.8589 mL
    5 mM 0.4372 mL 2.1859 mL 4.3718 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99%

    SDS (252 KB)

    COA (251 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1859 mL 10.9294 mL 21.8589 mL 54.6472 mL
    5 mM 0.4372 mL 2.1859 mL 4.3718 mL 10.9294 mL
    10 mM 0.2186 mL 1.0929 mL 2.1859 mL 5.4647 mL
    15 mM 0.1457 mL 0.7286 mL 1.4573 mL 3.6431 mL
    20 mM 0.1093 mL 0.5465 mL 1.0929 mL 2.7324 mL
    25 mM 0.0874 mL 0.4372 mL 0.8744 mL 2.1859 mL
    30 mM 0.0729 mL 0.3643 mL 0.7286 mL 1.8216 mL
    40 mM 0.0546 mL 0.2732 mL 0.5465 mL 1.3662 mL
    50 mM 0.0437 mL 0.2186 mL 0.4372 mL 1.0929 mL
    60 mM 0.0364 mL 0.1822 mL 0.3643 mL 0.9108 mL
    80 mM 0.0273 mL 0.1366 mL 0.2732 mL 0.6831 mL
    100 mM 0.0219 mL 0.1093 mL 0.2186 mL 0.5465 mL
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    DAPI dilactate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DAPI dilactate28718-91-44',6-Diamidino-2-phenylindole dilactateFluorescent DyeNucleic acid dyesNucleiCell cycledsDNAInhibitorinhibitorinhibit

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