DAz-2 

DAz-2 is a sulfonic acid probe for living cells, which can directly detect sulfonic acid-modified proteins in living cells and is cell-permeable^[1].

DAz-2 sulfonic acid detection methods under different situations^[1]
1. Prepare 10 μM protein, or 50 μg cell lysate, or 200 μg protein lysate.
2. React with 50 or 100 μM H₂O₂ at room temperature for 20 minutes; then add 1 mM DAz-2 or 5% (v/v) DMSO and react at room temperature for 15 minutes.
3. Quench the reaction with 5 mM DTT and remove excess reagent with gel filtration.
4. Use 4-12% SDS-PAGE to separate proteins, transfer to PVDF membrane, and detect by WB of HRP-Streptavidin.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

195.22

C₉H₁₃N₃O₂

1176905-54-6

[N-]=[N+]=NCCCC1C(CC(CC1)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Leonard SE, et al. Mining the thiol proteome for sulfenic acid modifications reveals new targets for oxidation in cells. ACS Chem Biol. 2009 Sep 18;4(9):783-99.  [Content Brief]