2. Others
  3. Fluorescent Dye
  4. DCIA

DCIA is a fluorescent dye. DCIA contains a coumarin fluorophore and conjugates specifically with free cysteines in the protein.

For research use only. We do not sell to patients.

DCIA Chemical Structure

DCIA Chemical Structure

CAS No. : 76877-34-4

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5 mg USD 190 In-stock
10 mg USD 285 Get quote
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Based on 1 publication(s) in Google Scholar

DCIA Related Antibodies

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  • Purity & Documentation

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Description

DCIA is a fluorescent dye. DCIA contains a coumarin fluorophore and conjugates specifically with free cysteines in the protein[1].

Molecular Weight

490.33

Formula

C22H23IN2O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

454
Excitation (Ex)

344
SMILES

O=C(NC1=CC=C(C2=C(C)C3=CC=C(N(CC)CC)C=C3OC2=O)C=C1)CI
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (101.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0394 mL 10.1972 mL 20.3944 mL
5 mM 0.4079 mL 2.0394 mL 4.0789 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.25 mg/mL (2.55 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0394 mL 10.1972 mL 20.3944 mL 50.9861 mL
5 mM 0.4079 mL 2.0394 mL 4.0789 mL 10.1972 mL
10 mM 0.2039 mL 1.0197 mL 2.0394 mL 5.0986 mL
15 mM 0.1360 mL 0.6798 mL 1.3596 mL 3.3991 mL
20 mM 0.1020 mL 0.5099 mL 1.0197 mL 2.5493 mL
25 mM 0.0816 mL 0.4079 mL 0.8158 mL 2.0394 mL
30 mM 0.0680 mL 0.3399 mL 0.6798 mL 1.6995 mL
40 mM 0.0510 mL 0.2549 mL 0.5099 mL 1.2747 mL
50 mM 0.0408 mL 0.2039 mL 0.4079 mL 1.0197 mL
60 mM 0.0340 mL 0.1700 mL 0.3399 mL 0.8498 mL
80 mM 0.0255 mL 0.1275 mL 0.2549 mL 0.6373 mL
100 mM 0.0204 mL 0.1020 mL 0.2039 mL 0.5099 mL
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DCIA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DCIA76877-34-4Fluorescent DyeCoumarinFluorophoreCysteinesInhibitorinhibitorinhibit

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