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  4. DFAME

DFAME is a red fluorophore (Ex=508 nm, Em=641 nm). Beetroot and Corn are dimeric fluorogenic RNA aptamers that can bind to DFAME to form Beetroot-DFAME (Kd=460nM) and Corn-DFAME (Kd= 3600nM). Beetroot-DFAME (Kd=460nM) and Corn-DFAME can be used to form RNA assemblies in living cells. Creating RNA assemblies can be used for the study of RNA Nanostructures. DNA/RNA Nanostructures would be useful in cell and gene therapy (CGT) research.

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DFAME Chemical Structure

DFAME Chemical Structure

CAS No. : 1420815-55-9

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Based on 1 publication(s) in Google Scholar

DFAME Related Antibodies

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Description

DFAME is a red fluorophore (Ex=508 nm, Em=641 nm). Beetroot and Corn are dimeric fluorogenic RNA aptamers that can bind to DFAME to form Beetroot-DFAME (Kd=460nM) and Corn-DFAME (Kd= 3600nM). Beetroot-DFAME (Kd=460nM) and Corn-DFAME can be used to form RNA assemblies in living cells. Creating RNA assemblies can be used for the study of RNA Nanostructures. DNA/RNA Nanostructures would be useful in cell and gene therapy (CGT) research[1].

Molecular Weight

322.26

Formula

C15H12F2N2O4
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C(OC)/C=C\C1=N/C(C(N1C)=O)=C/C2=CC(F)=C(O)C(F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (310.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1031 mL 15.5154 mL 31.0308 mL
5 mM 0.6206 mL 3.1031 mL 6.2062 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1031 mL 15.5154 mL 31.0308 mL 77.5771 mL
5 mM 0.6206 mL 3.1031 mL 6.2062 mL 15.5154 mL
10 mM 0.3103 mL 1.5515 mL 3.1031 mL 7.7577 mL
15 mM 0.2069 mL 1.0344 mL 2.0687 mL 5.1718 mL
20 mM 0.1552 mL 0.7758 mL 1.5515 mL 3.8789 mL
25 mM 0.1241 mL 0.6206 mL 1.2412 mL 3.1031 mL
30 mM 0.1034 mL 0.5172 mL 1.0344 mL 2.5859 mL
40 mM 0.0776 mL 0.3879 mL 0.7758 mL 1.9394 mL
50 mM 0.0621 mL 0.3103 mL 0.6206 mL 1.5515 mL
60 mM 0.0517 mL 0.2586 mL 0.5172 mL 1.2930 mL
80 mM 0.0388 mL 0.1939 mL 0.3879 mL 0.9697 mL
100 mM 0.0310 mL 0.1552 mL 0.3103 mL 0.7758 mL
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DFAME Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DFAME1420815-55-9Fluorescent DyefluorophoreBeetroot Corndimeric fluorogenic aptamersRNA assembliesInhibitorinhibitorinhibit

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