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DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells.

For research use only. We do not sell to patients.

DFHO Chemical Structure

DFHO Chemical Structure

CAS No. : 1420815-34-4

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1 mg USD 90 In-stock
5 mg USD 220 In-stock
10 mg USD 350 In-stock
25 mg USD 740 In-stock
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Based on 1 publication(s) in Google Scholar

DFHO Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells[1][2].

IC50 & Target

Kd: 70 nM (Corn binds DFHO)[1]
In Vitro

Corn is tested in E. coli. Minimal fluorescence that is seen in DFHO-treated cells (20 μM) transformed with the empty vector. However robust yellow fluorescence is seen in E. coli expressing Corn[1].
Corn-DFHO exhibits markedly enhanced photostability in vitro. The Corn-DFHO complexes are imaged in mammalian cells. Since the U6 promoter is one of the three major Pol III promoters, Corn from the U6 promoter is expressed and replaced the majority of the U6 sequence with Corn, leaving a minimal 5′ capping element leader sequence. The majority of the cells expressed diffuse yellow fluorescence, most prominently in the cytoplasm)[1].
DFHO exhibits low background fluorescence and cytotoxicity, making it a potentially suitable fluorophore for activation by RNA aptamers and for imaging experiments in living cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DFHO Related Antibodies
Molecular Weight

281.21

Formula

C12H9F2N3O3
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

O=C(N1C)/C(N=C1/C=N/O)=C\C(C=C2F)=CC(F)=C2O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (355.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5561 mL 17.7803 mL 35.5606 mL
5 mM 0.7112 mL 3.5561 mL 7.1121 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5561 mL 17.7803 mL 35.5606 mL 88.9015 mL
5 mM 0.7112 mL 3.5561 mL 7.1121 mL 17.7803 mL
10 mM 0.3556 mL 1.7780 mL 3.5561 mL 8.8902 mL
15 mM 0.2371 mL 1.1854 mL 2.3707 mL 5.9268 mL
20 mM 0.1778 mL 0.8890 mL 1.7780 mL 4.4451 mL
25 mM 0.1422 mL 0.7112 mL 1.4224 mL 3.5561 mL
30 mM 0.1185 mL 0.5927 mL 1.1854 mL 2.9634 mL
40 mM 0.0889 mL 0.4445 mL 0.8890 mL 2.2225 mL
50 mM 0.0711 mL 0.3556 mL 0.7112 mL 1.7780 mL
60 mM 0.0593 mL 0.2963 mL 0.5927 mL 1.4817 mL
80 mM 0.0445 mL 0.2223 mL 0.4445 mL 1.1113 mL
100 mM 0.0356 mL 0.1778 mL 0.3556 mL 0.8890 mL
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DFHO Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DFHO1420815-34-4Fluorescent DyeCornyellowfluorescenceligandRNAdimerizationimagingliving cellsaptamerRFPInhibitorinhibitorinhibit

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