DfTat 

DfTat is a dimer of the prototypical cell-penetrating peptide TAT. DfTat can deliver small molecules, peptides and proteins into live cells with a particularly high efficiency. DfTat labeled with the rhodamine can be used as a tracer for easy detection^[1].

4076.83

C₁₇₈H₂₉₂N₇₄O₃₄S₂

2035480-78-3

Solid

Brown to black

630

560

Chain 1:Cys-{Lys(5-TAMRA)}-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-NH2; Chain 2:Cys-Lys{(5-TAMRA)}-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-NH2 (disulfide bridge:chain 1 Cys-1 to chain 2 Cys-1)

Chain 1:C-{Lys(5-TAMRA)}-RKKRRQRRRG-NH2;Chain 2:C-{Lys(5-TAMRA)}-RKKRRQRRRG-NH2 (disulfide bridge:chain 1 cys-1 to chain 2 cys-1)

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture and light, under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Najjar K, et al. Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT. J Vis Exp. 2015 Sep 2;(103):53175.  [Content Brief]