Digitoxigenin (Synonyms: Cerberigenin; Echujetin)

Cat. No.: HY-B2151 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

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Digitoxigenin is a steroid derivative commonly found in various plants, especially the foxglove plant (Digitalis purpurea). Digitoxigenin has unique chemical properties that make it an important precursor for the synthesis of cardiac glycosides, a group of drugs used to improve heart failure and certain types of arrhythmias. It works by inhibiting the sodium potassium ATPase pump, thereby increasing the force and efficiency of cardiac contractions.

For research use only. We do not sell to patients.

Digitoxigenin Chemical Structure

CAS No. : 143-62-4

Size	Price	Stock	Quantity
5 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 316 μM Compound: 5	Viability of human A549 cells by MTT assay after 24 hrs Viability of human A549 cells by MTT assay after 24 hrs	[PMID: 17595134]
A549	IC₅₀	0.24 μg/mL Compound: 17	Cytotoxicity against human A549 cells after 3 days by MTT assay Cytotoxicity against human A549 cells after 3 days by MTT assay	[PMID: 16252914]
A549	IC₅₀	0.62 μM Compound: 5	Inhibition of induction of ICAM1 expression in human A549 cells in presence of IL1-alpha after 1 hr Inhibition of induction of ICAM1 expression in human A549 cells in presence of IL1-alpha after 1 hr	[PMID: 17595134]
A549	IC₅₀	1.68 μM Compound: DGTN	Cytotoxicity against human A549 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human A549 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
BC	IC₅₀	8.95 μM Compound: 3	Cytotoxicity against human BC cells by SRB assay Cytotoxicity against human BC cells by SRB assay	[PMID: 16933890]
HCT-8	IC₅₀	2.48 μM Compound: DGTN	Cytotoxicity against human HCT8 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human HCT8 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
HepG2	IC₅₀	0.12 μg/mL Compound: 17	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 16252914]
Hs-578T	IC₅₀	1.022 μM Compound: digitoxigenin	Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human Hs578T cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
Hs-578T	IC₅₀	92.3 μM Compound: digitoxigenin	Cytotoxicity against human Hs578T cells at 40 uM after 72 hrs by CellTiter-Glo assay relative to untreated control Cytotoxicity against human Hs578T cells at 40 uM after 72 hrs by CellTiter-Glo assay relative to untreated control	[PMID: 22316168]
HT-1080	IC₅₀	1500 nM Compound: 12	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 14640513]
KB	IC₅₀	9.61 μM Compound: 3	Cytotoxicity against human KB cells by SRB assay Cytotoxicity against human KB cells by SRB assay	[PMID: 16933890]
LNCaP	IC₅₀	3.7 μM Compound: DGTN	Cytotoxicity against human LNCAP cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human LNCAP cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
MCF7	IC₅₀	0.6 μM Compound: 4, DTG	Antiproliferative activity against human MCF7 cells assessed as cell viability treated for 48 hrs followed by compound washout measured after 72 hrs by fluorometric CellTiter-Blue reagent assay Antiproliferative activity against human MCF7 cells assessed as cell viability treated for 48 hrs followed by compound washout measured after 72 hrs by fluorometric CellTiter-Blue reagent assay	[PMID: 21421322]
MCF7	IC₅₀	1.69 μg/mL Compound: 17	Cytotoxicity against human MCF7 cells after 3 days by MTT assay Cytotoxicity against human MCF7 cells after 3 days by MTT assay	[PMID: 16252914]
MCF7	IC₅₀	194.4 nM Compound: 2	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 16309315]
MCF7	IC₅₀	9.1 μM Compound: digitoxigenin	Cytotoxicity against human MCF7 cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human MCF7 cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
MDA-MB-231	IC₅₀	1.9 μg/mL Compound: 17	Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay	[PMID: 16252914]
MRC5	IC₅₀	1.16 μM Compound: DGTN	Cytotoxicity against human MRC5 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
NCI-H187	IC₅₀	9.77 μM Compound: 3	Cytotoxicity against human NCI-H187 cells by SRB assay Cytotoxicity against human NCI-H187 cells by SRB assay	[PMID: 16933890]
NCI-H460	IC₅₀	0.92 μM Compound: DGTN	Cytotoxicity against human H460 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human H460 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
PC-3	IC₅₀	2.41 μM Compound: DGTN	Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by SRB assay	[PMID: 30798081]
SK-BR-3	IC₅₀	4.42 μM Compound: digitoxigenin	Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human SK-BR-3 cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
T47D	IC₅₀	2.53 μM Compound: digitoxigenin	Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay Cytotoxicity against human T47D cells after 72 hrs by CellTiter-Glo assay	[PMID: 22316168]
TK-10	IC₅₀	76.5 nM Compound: 2	Cytotoxicity against human TK10 cells after 48 hrs by SRB assay Cytotoxicity against human TK10 cells after 48 hrs by SRB assay	[PMID: 16309315]
UACC-62	IC₅₀	348.8 nM Compound: 2	Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay	[PMID: 16309315]
Vero	CC₅₀	27.54 μM Compound: DGTN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 30798081]

In Vitro

Digitoxigenin is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

374.51

Formula

C₂₃H₃₄O₄

CAS No.

143-62-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O[C@@]12[C@@]3([H])[C@@](CC[C@@]1([C@@H](C(CO4)=CC4=O)CC2)C)([H])[C@@]5([C@](C[C@H](CC5)O)([H])CC3)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (333.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6702 mL	13.3508 mL	26.7016 mL
5 mM	0.5340 mL	2.6702 mL	5.3403 mL
10 mM	0.2670 mL	1.3351 mL	2.6702 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (246 KB) SDS (479 KB)

COA (253 KB) HNMR (258 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6702 mL	13.3508 mL	26.7016 mL	66.7539 mL
	5 mM	0.5340 mL	2.6702 mL	5.3403 mL	13.3508 mL
	10 mM	0.2670 mL	1.3351 mL	2.6702 mL	6.6754 mL
	15 mM	0.1780 mL	0.8901 mL	1.7801 mL	4.4503 mL
	20 mM	0.1335 mL	0.6675 mL	1.3351 mL	3.3377 mL
	25 mM	0.1068 mL	0.5340 mL	1.0681 mL	2.6702 mL
	30 mM	0.0890 mL	0.4450 mL	0.8901 mL	2.2251 mL
	40 mM	0.0668 mL	0.3338 mL	0.6675 mL	1.6688 mL
	50 mM	0.0534 mL	0.2670 mL	0.5340 mL	1.3351 mL
	60 mM	0.0445 mL	0.2225 mL	0.4450 mL	1.1126 mL
	80 mM	0.0334 mL	0.1669 mL	0.3338 mL	0.8344 mL
	100 mM	0.0267 mL	0.1335 mL	0.2670 mL	0.6675 mL

Digitoxigenin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Digitoxigenin143-62-4Cerberigenin EchujetinBiochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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