2. Enzyme
  3. Nuclease
  4. DNase I, Bovine pancreas

DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer.

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DNase I, Bovine pancreas Chemical Structure

DNase I, Bovine pancreas Chemical Structure

CAS No. : 9003-98-9

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Based on 8 publication(s) in Google Scholar

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DNase I, Bovine pancreas Related Antibodies

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Description

DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer[1].

In Vitro

Product Information
The activity of DNase Ⅰ is dependent on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, and others. A concentration of 5 mM Ca2+ can protect the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognize and cleave any site on either strand of DNA; whereas, in the presence of Mn2+, it can simultaneously recognize both strands of DNA and cleave at nearly identical sites. This product is extracted from bovine pancreas and operates optimally within a pH range of 7-8. It has a molecular weight of approximately 31 kDa and an isoelectric point of around 6.0.

Instructions
Inactivation or Inhibition of DNase I:
Dissolved DNase I can be inactivated by heating at 65℃ for 10 min. Phenol-chloroform extraction can also inactivate DNase I. Metal ion chelators, zinc ions at millimolar concentrations, 0.1% SDS, reducing agents such as DTT and β-mercaptoethanol, and salt concentrations above 50-100 mM all significantly inhibit DNase I.
Usage Method for Protein Extraction Experiments (for reference only):
1)Preparation of storage solution: 5-10 mg/mL dissolved in 0.15 M NaCl, stored in the refrigerator, it is recommended to be used within 1 week.
2)Reaction System: Add DNase I stock solution to the protein extraction buffer at a 1/100 volume ratio (to achieve a final concentration of 20 U/mL) and 1 M MgCl2 at a 1/100 volume ratio.
3)Reaction Conditions: Incubate at 37℃ for 30-60 min. Proceed with subsequent protein extraction experiments.

Notes
1.Once the solution is prepared, please store it in aliquots to avoid product failure caused by repeated freezing and thawing.
2.Since EDTA can chelate Ca2+ and Mg2+ required for enzyme activity, EDTA should be removed from the initial protein lysis buffer, as it will reduce the digestive capacity of DNase I.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DNase I, Bovine pancreas Related Antibodies
In Vivo

Deoxyribonuclease (0.1 U; i.p.; once daily for 3 days) inhibits liver metastasis, besides results in a greater prolongation of the survival period by combining surgical removal of the primary tumour mass[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c x DBA/2 mice with murine tumour L5178Y-ML cells subcutaneous injection[1]
Dosage: 0.1 U per mouse
Administration: Intravenous injection; 0.1 U; once daily for three days
Result: Reduced liver weight from 2.26 to 1.55 g, affected the intensity of liver metastasis, reduced the numbers and sizes of metastatic and affected tumour cell arrest.
Animal Model: Female BALB/c x DBA/2 mice with murine tumour and surgical removal of subcutaneous tumours[1]
Dosage: 0.1 U per mouse
Administration: Intravenous injection; 0.1 U; once daily for 3 days either before or after primary tumour removal
Result: Significantly prolongated survival after tumour cell inoculation compared to the untreated group.
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

[DNase I, Bovine pancreas]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 43.48 mg/mL (adjust pH to 3 with 1M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  Saline

    Solubility: 5 mg/mL; Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

SDS (251 KB)

COA (230 KB)

Handling Instructions (2659 KB)
References
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DNase I, Bovine pancreas Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DNase I, Bovine pancreas9003-98-9DNaseOthersdegradeDNAcleavageenzymecancerinflammationInhibitorinhibitorinhibit

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