1. Enzyme
  2. Nuclease
  3. DNase I, Bovine pancreas

DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer.

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DNase I, Bovine pancreas Chemical Structure

DNase I, Bovine pancreas Chemical Structure

CAS No. : 9003-98-9

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Based on 13 publication(s) in Google Scholar

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Description

DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer[1].

In Vitro

Product Information
The activity of DNase Ⅰ is dependent on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, and others.
In the presence of Mg2+, the enzyme can randomly recognize and cleave any site on either strand of DNA; whereas, in the presence of Mn2+, it can simultaneously recognize both strands of DNA and cleave at nearly identical sites. This product is extracted from bovine pancreas and operates optimally within a pH range of 7-8. It has a molecular weight of approximately 31 kDa and an isoelectric point of around 6.0.

Instructions
Inactivation or Inhibition of DNase I:
Dissolved DNase I can be inactivated by heating at 65℃ for 10 min. Phenol-chloroform extraction can also inactivate DNase I. Metal ion chelators, zinc ions at millimolar concentrations, 0.1% SDS, reducing agents such as DTT and β-mercaptoethanol, and salt concentrations above 50-100 mM all significantly inhibit DNase I.
Usage Method for Protein Extraction Experiments (for reference only):
1)Preparation of storage solution: 5-10 mg/mL dissolved in 0.15 M NaCl, stored in the refrigerator, it is recommended to be used within 1 week.
2)Reaction System: Add DNase I stock solution to the protein extraction buffer at a 1/100 volume ratio (to achieve a final concentration of 20 U/mL) and 1 M MgCl2 at a 1/100 volume ratio.
3)Reaction Conditions: Incubate at 37℃ for 30-60 min. Proceed with subsequent protein extraction experiments.

Notes
1.Once the solution is prepared, please store it in aliquots to avoid product failure caused by repeated freezing and thawing.
2.Since EDTA can chelate Ca2+ and Mg2+ required for enzyme activity, EDTA should be removed from the initial protein lysis buffer, as it will reduce the digestive capacity of DNase I.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Deoxyribonuclease (0.1 U; i.p.; once daily for 3 days) inhibits liver metastasis, besides results in a greater prolongation of the survival period by combining surgical removal of the primary tumour mass[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c x DBA/2 mice with murine tumour L5178Y-ML cells subcutaneous injection[1]
Dosage: 0.1 U per mouse
Administration: Intravenous injection; 0.1 U; once daily for three days
Result: Reduced liver weight from 2.26 to 1.55 g, affected the intensity of liver metastasis, reduced the numbers and sizes of metastatic and affected tumour cell arrest.
Animal Model: Female BALB/c x DBA/2 mice with murine tumour and surgical removal of subcutaneous tumours[1]
Dosage: 0.1 U per mouse
Administration: Intravenous injection; 0.1 U; once daily for 3 days either before or after primary tumour removal
Result: Significantly prolongated survival after tumour cell inoculation compared to the untreated group.
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

[DNase I, Bovine pancreas]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : < 0.1 mg/mL (insoluble)

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Purity & Documentation

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DNase I, Bovine pancreas Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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DNase I, Bovine pancreas
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HY-108882
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