Recombinant DNase I (RNase-free) 

Recombinant DNase I (RNase-free) is a recombinant deoxyribonuclease that degrades DNA. Recombinant DNase I is essential for limiting inflammatory responses and maintaining homeostasis^[1].

Product Information
DNase I is a non-specific endonuclease that can degrade double-stranded and single-stranded DNA and chromosomes. It works by hydrolyzing phosphodiester bonds to produce mononucleotides and oligonucleotides with 5'-phosphate groups and 3'-hydroxyl groups. The activity of this enzyme requires the participation of Mg²⁺ or Ca²⁺ ions, and the optimal pH is about 7.8. This product uses Pichia pastoris to recombinantly express DNase I from bovine pancreas, and removes RNase activity during the purification process. The storage buffer is 10 mM Tris, 2 mM CaCl2, 50% glycerol, pH 7.6

Remove DNA contamination from RNA
1. The reaction system contains: RNA 10 μg, 10x DNase I Reaction Buffer (100 mM Tris, 5 mM CaCl2, 25 mM MgCl2, pH 7.6) 10 μL, recombinant DNase I 2U, and make up the volume to 100 μL with water.
2. Incubate at 37℃ for 10 min. After the reaction is complete, add EDTA to a final concentration of 5 mM and incubate at 75℃ for 10 min to inactivate DNase I.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9003-98-9

Liquid

Colorless to light yellow

[Recombinant DNase I (RNase-free)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lauková L, et al. Deoxyribonucleases and Their Applications in Biomedicine. Biomolecules. 2020 Jul 11;10(7):1036.  [Content Brief]

  [2]. Sugihara S, et al. Deoxyribonuclease treatment prevents blood-borne liver metastasis of cutaneously transplanted tumour cells in mice. Br J Cancer. 1993 Jan;67(1):66-70.  [Content Brief]