2. Enzyme
  3. Nuclease

Nuclease

Nucleases can enzymatically digest DNA or RNA and are widely used in biological research. Category includes common Ribonuclease H, DNase I, etc.

Nucleases are mainly used for:

• Purification of proteins and specific nucleic acids

• Used to solve the problem of excessive DNA concentration

• Improve the viscosity problem during cell dissociation

Nuclease (24):

Cat. No. Product Name CAS No.  
  • HY-108882
    DNase I, Bovine pancreas 9003-98-9  
    DNase I (EC 3.1.21.1) is an enzyme that degrade DNA, it plays a key role in the cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis. Exogenous deoxyribonuclease shows beneficial effects in inflammatory diseases and cancer.
  • HY-129046
    RNase A, Bovine pancreas 9001-99-4  
    RNase A, Bovine pancreas (Ribonuclease A) cleaves the 3' end of RNA to pyrimidine and actively cleaves RNA at each pyrimidine residue. RNase A, bovine pancreas catalyzes the hydrolysis of single-stranded RNA in the absence of metal ions or cofactors.
  • HY-129046A
    RNase A (10mg/mL, DNase free)  
    RNase A (10mg/mL, DNase free) is a ribonuclease used to catalyze RNA degradation without containing DNase. RNase A is often employed in cell cycle assay.
  • HY-P2963
    Nuclease P1 54576-84-0  
    Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.
  • HY-P2863
    Deoxyribonuclease II 9025-64-3  
    Deoxyribonuclease II (DNase II) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3' phosphate and 5'-hydroxyl termini. Deoxyribonuclease II works best at acidic pH and is commonly used in biochemical research.
  • HY-129046C
    RNase B, Bovine Pancreas  
    RNase B, Bovine Pancreas is an N-glycosylated form of bovine pancreatic ribonuclease, which is structurally analogous to RNase A. RNase B, Bovine Pancreas promotes the folding of polypeptide chains and performs a chaperone-like function.
  • HY-E70381
    dsDNase  
    dsDNase is a highly specific double-strand DNA (dsDNA) endonuclease that quickly and efficiently removes contaminating DNA from PCR master mixes.
  • HY-129046D
    RNase A, Recombinant 9001-99-4  
    RNase A, Recombinant (Ribonuclease A, Recombinant) is an endonuclease that specifically hydrolyzes cytosine or uracil residues in RNA for DNA purification.
  • HY-108882A
    Recombinant DNase I (RNase-free) 9003-98-9  
    Recombinant DNase I (RNase-free) is a recombinant deoxyribonuclease that degrades DNA. Recombinant DNase I is essential for limiting inflammatory responses and maintaining homeostasis.
  • HY-131160
    Nuclease, Serratia marcescens 9025-65-4  
    Serratia marcescens nuclease (EC 3.1.30.2) is a nonspecific nuclease. Serratia marcescens nuclease has broad utility due to its potent digestive activity toward both DNA and RNA.
  • HY-P2754
    Micrococcal nuclease 9013-53-0  
    Micrococcal nuclease is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
  • HY-P3260
    5′-Nucleotidase, Microorganism 9027-73-0  
    5′-Nucleotidase, Microorganism (CD73) is an intrinsic membrane glycoprotein present as an ectoenzyme. 5′-Nucleotidase catalyzes hydrolysis of 5-nucleotides to their corresponding nucleosides.
  • HY-E70098
    RNase H2  
    RNase H2 is the predominant source of RNase H activity in mammalian and human cells. RNase H2 protects genome integrity. RNase H2 has been associated with ribonucleotide removal from genomic DNA in yeast and mouse, where it is required for embryonic development.
  • HY-108860
    Oncaspar 130167-69-0  
    Oncaspar (PEG-L-asparaginase; Pegasparaginase), a pegylated form of native Escherichia coli-derived L-asparaginase, breaks down the amino acid asparagine that are circulating in the bloodstream. Oncaspar plays an important role in acute lymphoblastic leukemia (ALL).
  • HY-P2773
    Nuclease S1, Aspergillus oryzae 37288-25-8  
    Nuclease S1, Aspergillus oryzae is a specific endonuclease that degrades single stranded DNA (ssDNA) and RNA. Nuclease S1 can also remove protruding single-strand ends from double-stranded DNA.
  • HY-108858
    Dornase alfa 143831-71-4  
    Dornase alfa (rhDNase) is a recombinant human deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA. Dornase alfa hydrolyzes the DNA present in sputum/mucus and reduces viscosity in the lungs, promoting improved clearance of secretions. Dornase alfa plays an important role in cystic fibrosis.
  • HY-E70350
    Ribonuclease T2, Aspergillus oryzae  
    Ribonuclease T2, Aspergillus oryzae is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on enhancing enzyme reaction kinetics, substrate selectivity, and activity under harsh conditions such as low or high pH. By introducing stimulus responsiveness to these enzyme modifications, dynamic control of activity is also possible.
  • HY-E70219
    SpCas9 D10A Nickase  
    SpCas9 D10A Nickase is a mutant of the Cas9 protein. SpCas9 D10A Nickase retains the function of a cleavage domain of Cas9 nuclease and specifically cleaves the target single strand to form a nick. SpCas9 D10A Nickase reduces off-target effects.
  • HY-E70220
    AsCas12a Nuclease  
    AsCas12a Nuclease is a CRISPR nuclease, and can specifically cutting double-stranded DNA. AsCas12a Nuclease can be used for gene edited study.
  • HY-E70414
    DNase B, Escherichia coli  
    DNase B, Escherichia coli is an enzyme that specializes in the degradation of DNA and can be extracted from E.coli.