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  3. EDTA dipotassium dihydrate

EDTA dipotassium dihydrate  (Synonyms: Ethylenediaminetetraacetic acid dipotassium dihydrate)

Cat. No.: HY-D0836 Purity: ≥99.0%
Handling Instructions Technical Support

EDTA dipotassium dihydrate is a metal ion chelator that forms stable and water-soluble chelates with a variety of metal ions. EDTA dipotassium is commonly used as a blood anticoagulant, which can effectively prevent blood coagulation and maintain the morphology and function of cells in blood samples. In the industrial field, EDTA dipotassium can be used to remove the adverse effects of metal ions and prevent oxidation and deterioration caused by metal ions.

For research use only. We do not sell to patients.

EDTA dipotassium dihydrate Chemical Structure

EDTA dipotassium dihydrate Chemical Structure

CAS No. : 25102-12-9

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Description

EDTA dipotassium dihydrate is a metal ion chelator that forms stable and water-soluble chelates with a variety of metal ions. EDTA dipotassium is commonly used as a blood anticoagulant, which can effectively prevent blood coagulation and maintain the morphology and function of cells in blood samples. In the industrial field, EDTA dipotassium can be used to remove the adverse effects of metal ions and prevent oxidation and deterioration caused by metal ions[1][2].

In Vitro

EDTA dipotassium dihydrate can be used as a blood anticoagulant for hematological testing.
The drawn blood sample can be directly injected into a vacuum blood collection tube containing EDTA dipotassium dihydrate (2mL). Immediately after collection (<15 minutes) and 4 hours later, the sample can be tested for blood routine using a MEK-6318J/K hematology analyzer to measure multiple parameters including white blood cells, red blood cells, platelets, etc.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

404.45

Formula

C10H18K2N2O10

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CN(CCN(CC(O[K])=O)CC(O)=O)CC(O[K])=O.O.O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (247.25 mM)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4725 mL 12.3625 mL 24.7249 mL
5 mM 0.4945 mL 2.4725 mL 4.9450 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥99.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.4725 mL 12.3625 mL 24.7249 mL 61.8123 mL
5 mM 0.4945 mL 2.4725 mL 4.9450 mL 12.3625 mL
10 mM 0.2472 mL 1.2362 mL 2.4725 mL 6.1812 mL
15 mM 0.1648 mL 0.8242 mL 1.6483 mL 4.1208 mL
20 mM 0.1236 mL 0.6181 mL 1.2362 mL 3.0906 mL
25 mM 0.0989 mL 0.4945 mL 0.9890 mL 2.4725 mL
30 mM 0.0824 mL 0.4121 mL 0.8242 mL 2.0604 mL
40 mM 0.0618 mL 0.3091 mL 0.6181 mL 1.5453 mL
50 mM 0.0494 mL 0.2472 mL 0.4945 mL 1.2362 mL
60 mM 0.0412 mL 0.2060 mL 0.4121 mL 1.0302 mL
80 mM 0.0309 mL 0.1545 mL 0.3091 mL 0.7727 mL
100 mM 0.0247 mL 0.1236 mL 0.2472 mL 0.6181 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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EDTA dipotassium dihydrate Related Classifications

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EDTA dipotassium dihydrate
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