2. PROTAC
  3. PROTAC Linkers
  4. EGNHS

EGNHS  (Synonyms: EGS crosslinker)

Cat. No.: HY-130458 Purity: 97.45%
COA Handling Instructions

Molarity Calculator

EGNHS is an alkyl/ether-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

EGNHS Chemical Structure

EGNHS Chemical Structure

CAS No. : 70539-42-3

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Based on 2 publication(s) in Google Scholar

EGNHS Related Antibodies

Top Publications Citing Use of Products

View All PROTAC Linkers Isoform Specific Products:

View All Isoforms
PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

EGNHS is an alkyl/ether-based PROTAC linker that can be used in the synthesis of PROTACs[1].

IC50 & Target

Alkyl/ether

 

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EGNHS Related Antibodies
Molecular Weight

456.36

Formula

C18H20N2O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCOC(CCC(ON1C(CCC1=O)=O)=O)=O)CCC(ON2C(CCC2=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (219.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1913 mL 10.9563 mL 21.9125 mL
5 mM 0.4383 mL 2.1913 mL 4.3825 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 97.45%

COA (246 KB) HNMR (125 KB) LCMS (239 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1913 mL 10.9563 mL 21.9125 mL 54.7813 mL
5 mM 0.4383 mL 2.1913 mL 4.3825 mL 10.9563 mL
10 mM 0.2191 mL 1.0956 mL 2.1913 mL 5.4781 mL
15 mM 0.1461 mL 0.7304 mL 1.4608 mL 3.6521 mL
20 mM 0.1096 mL 0.5478 mL 1.0956 mL 2.7391 mL
25 mM 0.0877 mL 0.4383 mL 0.8765 mL 2.1913 mL
30 mM 0.0730 mL 0.3652 mL 0.7304 mL 1.8260 mL
40 mM 0.0548 mL 0.2739 mL 0.5478 mL 1.3695 mL
50 mM 0.0438 mL 0.2191 mL 0.4383 mL 1.0956 mL
60 mM 0.0365 mL 0.1826 mL 0.3652 mL 0.9130 mL
80 mM 0.0274 mL 0.1370 mL 0.2739 mL 0.6848 mL
100 mM 0.0219 mL 0.1096 mL 0.2191 mL 0.5478 mL
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EGNHS Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EGNHS70539-42-3EGS crosslinkerPROTAC LinkersInhibitorinhibitorinhibit

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