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  3. FAM azide, 6-isomer

FAM azide, 6-isomer  (Synonyms: 6-FAM azide)

Cat. No.: HY-D1314 Purity: 99.21%
SDS COA Handling Instructions

FAM azide, 6-isomer (6-FAM azide), a fluorescent dye, is a click chemistry reagent containing an azide group. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

FAM azide, 6-isomer Chemical Structure

FAM azide, 6-isomer Chemical Structure

CAS No. : 1386385-76-7

Size Price Stock Quantity
1 mg USD 75 In-stock
5 mg USD 100 In-stock
10 mg USD 140 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

FAM azide, 6-isomer (6-FAM azide), a fluorescent dye, is a click chemistry reagent containing an azide group[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

Molecular Weight

458.42

Formula

C24H18N4O6

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

OC(C=C1OC2=CC(O)=CC=C23)=CC=C1C3(O4)C5=CC(C(NCCCN=[N+]=[N-])=O)=CC=C5C4=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (218.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Methanol : 5 mg/mL (10.91 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1814 mL 10.9070 mL 21.8141 mL
5 mM 0.4363 mL 2.1814 mL 4.3628 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.21%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Methanol / DMSO 1 mM 2.1814 mL 10.9070 mL 21.8141 mL 54.5351 mL
5 mM 0.4363 mL 2.1814 mL 4.3628 mL 10.9070 mL
10 mM 0.2181 mL 1.0907 mL 2.1814 mL 5.4535 mL
DMSO 15 mM 0.1454 mL 0.7271 mL 1.4543 mL 3.6357 mL
20 mM 0.1091 mL 0.5454 mL 1.0907 mL 2.7268 mL
25 mM 0.0873 mL 0.4363 mL 0.8726 mL 2.1814 mL
30 mM 0.0727 mL 0.3636 mL 0.7271 mL 1.8178 mL
40 mM 0.0545 mL 0.2727 mL 0.5454 mL 1.3634 mL
50 mM 0.0436 mL 0.2181 mL 0.4363 mL 1.0907 mL
60 mM 0.0364 mL 0.1818 mL 0.3636 mL 0.9089 mL
80 mM 0.0273 mL 0.1363 mL 0.2727 mL 0.6817 mL
100 mM 0.0218 mL 0.1091 mL 0.2181 mL 0.5454 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FAM azide, 6-isomer
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