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  3. Fluorescent Dye
  4. Coumarin 6

Coumarin 6, a fluorescent dye, is used as a fluorescent probe in a microparticle drug delivery system to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems (λexc=450 nm, λem=505 nm).

For research use only. We do not sell to patients.

Coumarin 6 Chemical Structure

Coumarin 6 Chemical Structure

CAS No. : 38215-36-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
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Based on 7 publication(s) in Google Scholar

Coumarin 6 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

Coumarin 6, a fluorescent dye, is used as a fluorescent probe in a microparticle drug delivery system to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems (λexc=450 nm, λem=505 nm)[1][2][3].

In Vitro

Coumarin 6 labeled HPMC nanoparticles[3]
(1) Dissolve Coumarin 6 in DMF to prepare a 5 mg/mL organic phase solution.
(2) Dissolve hydroxypropyl methyl cellulose (HPMC) in HBSS to prepare a 50 μg/mL aqueous solution.
(3) Add the organic solution to the aqueous solution using a MasterFlex L/S pump (volume ratio of organic solution to aqueous solution is 1:100) with an injection rate of 36 mL/min.
(4) Prepare 70 nm nanoparticles by stirring at room temperature for 5 min at a stirring speed of 9 mL/min.
(5) Add 2 μg/mL nanoparticles to the MDCKII cell culture dish and incubate in the dark for 5 min, 15 min, 30 min and 60 min.
(6) Add cold PBS buffer to stop the uptake of nanoparticles by MDCKII cells and clean with HBSS.
(7) Use a fluorescence microscope to observe the uptake of nanoparticles by MDCKII cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coumarin 6 Related Antibodies
Molecular Weight

350.43

Formula

C20H18N2O2S
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

505
Excitation (Ex)

450
SMILES

O=C1C(C2=NC3=CC=CC=C3S2)=CC4=CC=C(N(CC)CC)C=C4O1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

THF : 12.5 mg/mL (35.67 mM; Need ultrasonic)

DMSO : 5 mg/mL (14.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8536 mL 14.2682 mL 28.5364 mL
5 mM 0.5707 mL 2.8536 mL 5.7073 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.93%

COA (250 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / THF 1 mM 2.8536 mL 14.2682 mL 28.5364 mL 71.3409 mL
5 mM 0.5707 mL 2.8536 mL 5.7073 mL 14.2682 mL
10 mM 0.2854 mL 1.4268 mL 2.8536 mL 7.1341 mL
THF 15 mM 0.1902 mL 0.9512 mL 1.9024 mL 4.7561 mL
20 mM 0.1427 mL 0.7134 mL 1.4268 mL 3.5670 mL
25 mM 0.1141 mL 0.5707 mL 1.1415 mL 2.8536 mL
30 mM 0.0951 mL 0.4756 mL 0.9512 mL 2.3780 mL
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Coumarin 6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coumarin 638215-36-0Coumarin6Coumarin-6Fluorescent DyeInhibitorinhibitorinhibit

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