Gemcitabine-O-Si(di-iso)-O-Mc

Name: Gemcitabine-O-Si(di-iso)-O-Mc
Brand: MedChemExpress
SKU: HY-130812
Rating: 4.5 (1 reviews)

Cat. No.: HY-130812 Purity: 98.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Gemcitabine-O-Si(di-iso)-O-Mc is a agent-linker conjugate for ADC with potent antitumor activity by using Gemcitabine (a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent; HY-17026), linked via the ADC linker.

For research use only. We do not sell to patients.

Gemcitabine-O-Si(di-iso)-O-Mc Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1500	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Antibody drug conjugates (ADC) couple therapeutic monoclonal antibodies (mAb) with potent toxins through a linker that is stable within systemic circulation, but cleaves within the target cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

558.65

Formula

C₂₄H₃₆F₂N₄O₇Si

Appearance

Solid

Color

Off-white to light yellow

SMILES

NC(C=CN1[C@H]2C(F)([C@@H]([C@H](O2)CO[Si](C(C)C)(OCCCCCN3C(C=CC3=O)=O)C(C)C)O)F)=NC1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (179.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7900 mL	8.9501 mL	17.9003 mL
5 mM	0.3580 mL	1.7900 mL	3.5801 mL
10 mM	0.1790 mL	0.8950 mL	1.7900 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.48 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (247 KB) HNMR (191 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Mathew C. Finniss, et al. A versatile acid-labile linker for antibody–drug conjugate. MedChemComm, Issue 9, 2014.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7900 mL	8.9501 mL	17.9003 mL	44.7507 mL
	5 mM	0.3580 mL	1.7900 mL	3.5801 mL	8.9501 mL
	10 mM	0.1790 mL	0.8950 mL	1.7900 mL	4.4751 mL
	15 mM	0.1193 mL	0.5967 mL	1.1934 mL	2.9834 mL
	20 mM	0.0895 mL	0.4475 mL	0.8950 mL	2.2375 mL
	25 mM	0.0716 mL	0.3580 mL	0.7160 mL	1.7900 mL
	30 mM	0.0597 mL	0.2983 mL	0.5967 mL	1.4917 mL
	40 mM	0.0448 mL	0.2238 mL	0.4475 mL	1.1188 mL
	50 mM	0.0358 mL	0.1790 mL	0.3580 mL	0.8950 mL
	60 mM	0.0298 mL	0.1492 mL	0.2983 mL	0.7458 mL
	80 mM	0.0224 mL	0.1119 mL	0.2238 mL	0.5594 mL
	100 mM	0.0179 mL	0.0895 mL	0.1790 mL	0.4475 mL