Nos3 - nitric oxide synthase 3, endothelial cell Gene

Mus musculus

Also known as eNOS; Nos-3; ecNOS; 2310065A03Rik

Gene ID: 18127 | Gene type: protein coding

About Nos3

Chromosome 5: 24,569,808-24,589,472 forward strand.GRCm39:CM000998.3

This gene has 2 transcripts (splice variants), 114 orthologues, 5 paralogues and is associated with 243 phenotypes. Broad expression in subcutaneous fat pad adult (RPKM 22.), mammary gland adult (RPKM 2.1) and 26 other tissues.

Summary

Enables nitric-oxide synthase activity and scaffold protein binding activity. Involved in several processes, including heart development; negative regulation of muscle hyperplasia; and smooth muscle hyperplasia. Acts upstream of or within several processes, including lipopolysaccharide-mediated signaling pathway; nitric oxide biosynthetic process; and regulation of metal ion transport. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; hemolymphoid system gland; and nervous system. Used to study aortic valve disease; essential hypertension; and persistent fetal circulation syndrome. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease (multiple); artery disease (multiple); eye disease (multiple); reproductive organ Cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Apr 2022]

Nos3 Products(1)

mRNA	Protein	Name
NM_008713.4	NP_032739.3	nitric oxide synthase, endothelial

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables nitric-oxide synthase activity	IMP IMP: Inferred from mutant phenotype	15647265	MGI
enables protein binding	IPI IPI: Inferred from physical interaction	22081021	MGI
enables scaffold protein binding	IPI IPI: Inferred from physical interaction	25017399	MGI

Biological Process GO Annotation	Evidence	Reference	Source
acts upstream of or within angiogenesis	IMP IMP: Inferred from mutant phenotype	15016731	MGI
involved in aortic valve morphogenesis	IGI IGI: Inferred from genetic interaction	27107132	MGI
involved in aortic valve morphogenesis	IMP IMP: Inferred from mutant phenotype	10821808	MGI
involved in blood vessel diameter maintenance	IMP IMP: Inferred from mutant phenotype	9466966	MGI
involved in blood vessel remodeling	IMP IMP: Inferred from mutant phenotype	9466966	MGI
acts upstream of calcium ion transport	IMP IMP: Inferred from mutant phenotype	12074580	MGI
involved in endocardial cushion morphogenesis	IGI IGI: Inferred from genetic interaction	27107132	MGI
acts upstream of establishment of localization in cell	IMP IMP: Inferred from mutant phenotype	12074580	MGI
involved in homeostasis of number of cells within a tissue	IGI IGI: Inferred from genetic interaction	27107132	MGI
acts upstream of or within in utero embryonic development	IMP IMP: Inferred from mutant phenotype	11732991	MGI
acts upstream of or within lipopolysaccharide-mediated signaling pathway	IMP IMP: Inferred from mutant phenotype	15647265	MGI
acts upstream of or within lung development	IMP IMP: Inferred from mutant phenotype	15016731	MGI
involved in negative regulation of biomineral tissue development	IMP IMP: Inferred from mutant phenotype	23583836	MGI
acts upstream of or within negative regulation of calcium ion transport	IMP IMP: Inferred from mutant phenotype	12074580	MGI
involved in negative regulation of cell population proliferation	IMP IMP: Inferred from mutant phenotype	9466966	MGI
acts upstream of or within negative regulation of hydrolase activity	IMP IMP: Inferred from mutant phenotype	12074580	MGI
involved in negative regulation of muscle hyperplasia	IMP IMP: Inferred from mutant phenotype	9466966	MGI
acts upstream of or within negative regulation of potassium ion transport	IMP IMP: Inferred from mutant phenotype	12074580	MGI
acts upstream of or within negative regulation of smooth muscle cell proliferation	IMP IMP: Inferred from mutant phenotype	20554900	MGI
acts upstream of or within nitric oxide biosynthetic process	IMP IMP: Inferred from mutant phenotype	15647265	MGI
acts upstream of or within ovulation from ovarian follicle	IMP IMP: Inferred from mutant phenotype	11779605	MGI
involved in positive regulation of angiogenesis	IMP IMP: Inferred from mutant phenotype	9616228	MGI
acts upstream of positive effect positive regulation of gene expression	IGI IGI: Inferred from genetic interaction	23583836	MGI
acts upstream of positive effect positive regulation of gene expression	IMP IMP: Inferred from mutant phenotype	23583836	MGI
acts upstream of potassium ion transport	IMP IMP: Inferred from mutant phenotype	12074580	MGI
involved in pulmonary valve morphogenesis	IGI IGI: Inferred from genetic interaction	27107132	MGI
involved in regulation of nervous system process	IGI IGI: Inferred from genetic interaction	29353861	MGI
acts upstream of or within regulation of sodium ion transport	IMP IMP: Inferred from mutant phenotype	12074580	MGI
acts upstream of or within regulation of the force of heart contraction by chemical signal	IGI IGI: Inferred from genetic interaction	18951881	MGI
involved in smooth muscle hyperplasia	IMP IMP: Inferred from mutant phenotype	9466966	MGI
involved in ventricular septum morphogenesis	IGI IGI: Inferred from genetic interaction	27107132	MGI

Cellular Component GO Annotation	Evidence	Reference	Source
located in cytoplasm	IDA IDA: Inferred from direct assay	11732991	MGI
located in nucleus	IDA IDA: Inferred from direct assay	11732991	MGI

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Nos3 Protein Structure

NOS_oxygenase_euk

Flavodoxin_1

FNR_like

0
200
400
600
800
1000
1202 a.a.

Protein Preferred Names

Protein Names

nitric oxide synthase, endothelial

EC-NOS	NOS type III
NOSIII	cNOS
constitutive NOS	endothelial NOS
endothelial nitric oxide synthase

Nos3 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P81191	Phospho-eNOS (Ser1177) Antibody	WB, ELISA, IHC-P, IHC-F, FC, ICC/IF	Human, Mouse

Related Agents

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-18732A	L-NMMA acetate	Inhibitor	≥99.0%	Phase 4

Pre-clinical Phase

Bioactive Molecules (4)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS09441	Nos3 Mouse Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown
HY-N8761	Kihadanin A	Inhibitor	≥97.0%	Unkown
HY-RS09440	NOS3 Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown
HY-RS09442	Nos3 Rat Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Nos3	NCBI	NCBI:4846

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