Glofitamab  (Synonyms: RO7082859)

Glofitamab (RO7082859) is a T-cell-engaging bispecific antibody possessing a novel 2:1 structure with bivalency for CD20 on B cells and monovalency for CD3 on T cells. Glofitamab leads to T-cell activation, proliferation, and tumor cell killing upon binding to CD20 on malignant cells. Glofitamab induces durable complete remissions in relapsed or refractory B-Cell non-Hodgkin lymphoma (B-NHL)^[1][2][3][4].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Humanized

Glofitamab (0.1 µg/mL, 48 h) stimulates the expression of 4-1BB (CD137) on CD8+ tumor-infiltrating lymphocyte (TILs) in the lymphoma tumor microenvironment (TMEs) and augmented diffuse large B cell lymphoma (DLBCL) cell death combined with either FAP-IL2v (HY-P99902) or FAP-4-1BBL^[4].
Glofitamab (0.2-200 ng/mL, 16 h)-mediated DLBCL cell killing requires the formation of stable T effector cell-tumor cell synapses^[5].
Glofitamab has no effect on the transient reduction in T-lymphocyte subsets, NK cells and monocytes by Obinutuzumab (HY-P9910) pretreatment (Gpt)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Glofitamab (0.3-100 μg/kg, i.v., a single dose) induces a dose-dependent increase in cytokine levels (IL-1Ra, IL-2, IL-5, IL-6, IL-8, IL-10, G-CSF, IFN-γ and TNF-α) in Cynomolgus Monkeys^[3].
Glofitamab (0.5 mg/kg, i.v., a single dose) induces early and lasting T cell engagement and activity in mice^[5].

Pharmacokinetic parameters of Glofitamab (i.v., a single dose) in cynomolgus monkeys with or without Obinutuzumab (Gpt: 50 mg/kg) (MeSt: Mechanistic Study; MTD: Maximum Tolerated Dose)^[3]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2229047-91-8

Liquid

Colorless to light yellow

[Glofitamab]

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

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  IgG1-kappa/lambda with domain crossover, trivalent

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  Flow cytometry analysis of Jurkat cells labelling CD3 with Glofitamab at 200 nM (Red). Goat anti human IgG Fc (Alexa Fluor488) was used as the secondary antibody at 1/1000 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).
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  Flow cytometry analysis of Raji cells labelling CD20 with Glofitamab at 20 nM (Red). Goat anti human IgG Fc (Alexa Fluor488) was used as the secondary antibody at 1/1000 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).

• [1]. Hutchings M, et al. Glofitamab, a Novel, Bivalent CD20-Targeting T-Cell-Engaging Bispecific Antibody, Induces Durable Complete Remissions in Relapsed or Refractory B-Cell Lymphoma: A Phase I Trial. J Clin Oncol. 2021;39(18):1959-1970.  [Content Brief]

  [2]. Bröske AE, et al. Pharmacodynamics and molecular correlates of response to glofitamab in relapsed/refractory non-Hodgkin lymphoma. Blood Adv. 2022;6(3):1025-1037.  [Content Brief]

  [3]. Frances N, et al. Novel in Vivo and in Vitro Pharmacokinetic/Pharmacodynamic-Based Human Starting Dose Selection for Glofitamab. J Pharm Sci. 2022 Apr;111(4):1208-1218.  [Content Brief]

  [4]. Apollonio B, et al. Tumor-activated lymph node fibroblasts suppress T cell function in diffuse large B cell lymphoma. J Clin Invest. 2023 Jul 3;133(13):e166070.  [Content Brief]

  [5]. Cremasco F, et al. Cross-linking of T cell to B cell lymphoma by the T cell bispecific antibody CD20-TCB induces IFNγ/CXCL10-dependent peripheral T cell recruitment in humanized murine model. PLoS One. 2021 Jan 6;16(1):e0241091.  [Content Brief]