2. Metabolic Enzyme/Protease Epigenetics
  3. Endogenous Metabolite MicroRNA
  4. Guanosine-5'-triphosphate disodium salt

Guanosine-5'-triphosphate disodium salt  (Synonyms: 5'-GTP disodium salt)

Cat. No.: HY-W010737 Purity: 99.75%
SDS COA Handling Instructions Technical Support

Guanosine 5'-triphosphate (5'-GTP) trisodium salt is a G protein (G proteins) signaling activator and a high-energy precursor in the biosynthesis of nucleotide units in DNA and RNA. Guanosine 5'-triphosphate trisodium salt can promote myogenic cell differentiation by upregulating miRNA (miR133a, miR133b) and myogenic regulatory factor expression, and by inducing human myogenic precursor cells to release exosomes containing guanosine molecules. Guanosine-5'-triphosphate disodium salt holds promise for research in biosynthesis and skeletal muscle regeneration.

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Guanosine-5'-triphosphate disodium salt Chemical Structure

Guanosine-5'-triphosphate disodium salt Chemical Structure

CAS No. : 56001-37-7

Size Price Stock Quantity
Solvent
250 mg (100 mM * 4.41 mL in Water) In-stock
Solvent
500 mg (100 mM * 8.82 mL in Water) In-stock
Solvent
1 g (100 mM * 17.63 mL in Water) In-stock
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Based on 6 publication(s) in Google Scholar

Other Forms of Guanosine-5'-triphosphate disodium salt:

Guanosine-5'-triphosphate disodium salt Related Antibodies

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  • Purity & Documentation

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Description

Guanosine 5'-triphosphate (5'-GTP) trisodium salt is a G protein (G proteins) signaling activator and a high-energy precursor in the biosynthesis of nucleotide units in DNA and RNA. Guanosine 5'-triphosphate trisodium salt can promote myogenic cell differentiation by upregulating miRNA (miR133a, miR133b) and myogenic regulatory factor expression, and by inducing human myogenic precursor cells to release exosomes containing guanosine molecules. Guanosine-5'-triphosphate disodium salt holds promise for research in biosynthesis and skeletal muscle regeneration[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Guanosine-5'-triphosphate disodium salt (500 μM, 24 hours) significantly promoted the upregulation of miR133a, miR133b, and myogenic regulatory factors Pax7, MyoD, and Myogenin in differentiated human myogenic precursor cells (MPCs)[2].
Guanosine-5'-triphosphate disodium salt (5'-GTP trisodium salt) (500 μM, 24 h) can induce the release of exosomes from human myogenic progenitor cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Guanosine-5'-triphosphate disodium salt Related Antibodies

Western Blot Analysis[2]

Cell Line: Human myogenic precursor cells (MPCs)
Concentration: 500 μM
Incubation Time: 24 h
Result: led to a significant upregulation of miR133a and miR133b and induced human myogenic precursor cells to release exosomes stuffed with guanosine based molecules (mainly guanosine) in the extracellular milieu.
Clinical Trial
Molecular Weight

567.14

Formula

C10H14N5Na2O14P3
CAS No.
Appearance

Liquid

Color

Colorless to off-white

SMILES

O=C1C2=C(N(C=N2)[C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O[Na])=O)=O)(O[Na])=O)O3)N=C(N)N1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, protect from light, 2 years
Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (110.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7632 mL 8.8162 mL 17.6323 mL
5 mM 0.3526 mL 1.7632 mL 3.5265 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (176.32 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.75%

SDS (393 KB)

COA (250 KB) HNMR (285 KB) CNMR (286 KB) RP-HPLC (258 KB) MS (80 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.7632 mL 8.8162 mL 17.6323 mL 44.0808 mL
5 mM 0.3526 mL 1.7632 mL 3.5265 mL 8.8162 mL
10 mM 0.1763 mL 0.8816 mL 1.7632 mL 4.4081 mL
15 mM 0.1175 mL 0.5877 mL 1.1755 mL 2.9387 mL
20 mM 0.0882 mL 0.4408 mL 0.8816 mL 2.2040 mL
25 mM 0.0705 mL 0.3526 mL 0.7053 mL 1.7632 mL
30 mM 0.0588 mL 0.2939 mL 0.5877 mL 1.4694 mL
40 mM 0.0441 mL 0.2204 mL 0.4408 mL 1.1020 mL
50 mM 0.0353 mL 0.1763 mL 0.3526 mL 0.8816 mL
60 mM 0.0294 mL 0.1469 mL 0.2939 mL 0.7347 mL
80 mM 0.0220 mL 0.1102 mL 0.2204 mL 0.5510 mL
100 mM 0.0176 mL 0.0882 mL 0.1763 mL 0.4408 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Guanosine-5'-triphosphate disodium salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Guanosine-5'-triphosphate disodium56001-37-75'-GTP disodiumEndogenous MetaboliteMicroRNAmiRNAHuman myogenic precursor cells (MPCs)Proliferative functionExosomesInhibitorinhibitorinhibit

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