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  4. H-D-Phe-Pip-Arg-pNA dihydrochloride

H-D-Phe-Pip-Arg-pNA dihydrochloride  (Synonyms: S-2238 dihydrochloride)

Cat. No.: HY-123275C
Handling Instructions

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H-D-Phe-Pip-Arg-pNA (S-2238) dihydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA dihydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA dihydrochloride is sensitive, accurate, and easy to perform.

For research use only. We do not sell to patients.

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H-D-Phe-Pip-Arg-pNA dihydrochloride Chemical Structure

H-D-Phe-Pip-Arg-pNA dihydrochloride Chemical Structure

CAS No. : 62354-65-8

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Other Forms of H-D-Phe-Pip-Arg-pNA dihydrochloride:

H-D-Phe-Pip-Arg-pNA dihydrochloride Related Antibodies

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Description

H-D-Phe-Pip-Arg-pNA (S-2238) dihydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA dihydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA dihydrochloride is sensitive, accurate, and easy to perform[1][2].

Molecular Weight

625.55

Formula

C27H38Cl2N8O5
CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (199.82 mM; Need ultrasonic)

DMSO : 125 mg/mL (199.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5986 mL 7.9930 mL 15.9859 mL
5 mM 0.3197 mL 1.5986 mL 3.1972 mL
View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.5986 mL 7.9930 mL 15.9859 mL 39.9648 mL
5 mM 0.3197 mL 1.5986 mL 3.1972 mL 7.9930 mL
10 mM 0.1599 mL 0.7993 mL 1.5986 mL 3.9965 mL
15 mM 0.1066 mL 0.5329 mL 1.0657 mL 2.6643 mL
20 mM 0.0799 mL 0.3996 mL 0.7993 mL 1.9982 mL
25 mM 0.0639 mL 0.3197 mL 0.6394 mL 1.5986 mL
30 mM 0.0533 mL 0.2664 mL 0.5329 mL 1.3322 mL
40 mM 0.0400 mL 0.1998 mL 0.3996 mL 0.9991 mL
50 mM 0.0320 mL 0.1599 mL 0.3197 mL 0.7993 mL
60 mM 0.0266 mL 0.1332 mL 0.2664 mL 0.6661 mL
80 mM 0.0200 mL 0.0999 mL 0.1998 mL 0.4996 mL
100 mM 0.0160 mL 0.0799 mL 0.1599 mL 0.3996 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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H-D-Phe-Pip-Arg-pNA dihydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-D-Phe-Pip-Arg-pNA62354-65-8S-2238S2238S 2238Fluorescent Dyechromogenicsubstratefibrinogenthrombinantithrombin-heparincofactorAT-IIIInhibitorinhibitorinhibit

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