2. PI3K/Akt/mTOR Stem Cell/Wnt MAPK/ERK Pathway
  3. PI3K ERK
  4. Hirsutenone

Hirsutenone is an active botanical diarylheptanoid present in Alnus species and exhibits many biological activities, including anti-inflammatory, anti-tumor promoting and anti-atopic dermatitis effects. Hirsutenone attenuates adipogenesis by binding directly to PI3K and ERK1 in a non-ATP competitive manner. Hirsutenone can be used for the study of obesity.

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Hirsutenone Chemical Structure

Hirsutenone Chemical Structure

CAS No. : 41137-87-5

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Based on 1 publication(s) in Google Scholar

Hirsutenone Related Antibodies

Top Publications Citing Use of Products

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

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Description

Hirsutenone is an active botanical diarylheptanoid present in Alnus species and exhibits many biological activities, including anti-inflammatory, anti-tumor promoting and anti-atopic dermatitis effects. Hirsutenone attenuates adipogenesis by binding directly to PI3K and ERK1 in a non-ATP competitive manner. Hirsutenone can be used for the study of obesity[1].

Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
220 μM
Compound: 42
Cytotoxicity against HEK293 cells after 16 hrs by alamar blue assay
Cytotoxicity against HEK293 cells after 16 hrs by alamar blue assay
[PMID: 20004045]
HSC-T6 IC50
14 μM
Compound: 2
Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay
Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay
[PMID: 21504848]
RAW264.7 IC50
> 100 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis
[PMID: 33422907]
RAW264.7 IC50
> 100 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NF-kappaB activation incubated for 24 hrs by SEAP microplate reader analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NF-kappaB activation incubated for 24 hrs by SEAP microplate reader analysis
[PMID: 33422907]
RAW264.7 IC50
> 100 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production preincubated for 1 hr followed by LPS stimulation and measured after 18 hrs by immunoassay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production preincubated for 1 hr followed by LPS stimulation and measured after 18 hrs by immunoassay
[PMID: 33422907]
RAW264.7 IC50
0.8 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess method
[PMID: 33422907]
RAW264.7 IC50
14.1 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess method
[PMID: 33422907]
RAW264.7 IC50
25 μg/mL
Compound: 34
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of IFN-gamma and LPS-induced COX2 production preincubated for 2 hrs followed by IFN-gamma and LPS stimulation and measured after 16 hrs by spectrophotometric analysis
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of IFN-gamma and LPS-induced COX2 production preincubated for 2 hrs followed by IFN-gamma and LPS stimulation and measured after 16 hrs by spectrophotometric analysis
[PMID: 33422907]
RAW264.7 IC50
27.2 μg/mL
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma and LPS-induced NO production incubated for 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma and LPS-induced NO production incubated for 24 hrs by Griess assay
[PMID: 33422907]
RAW264.7 IC50
39 μM
Compound: 34
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability by MTT assay
[PMID: 33422907]
RAW264.7 IC50
5 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 0.5 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 0.5 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
[PMID: 33422907]
RAW264.7 IC50
5.8 μM
Compound: 34
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based analysis
[PMID: 33422907]
In Vitro

Hirsutenone (0-100 μM; 48 hours) inhibits adipogenesis in 3T3-L1 preadipocytes and exhibits non-toxicity at 20-100 μM[1].
Hirsutenone (0-100 μM; 48 hours) attenuates MDI-induced lipid accumulation in 3T3-L1 preadipocytes in a dose-dependent manner. In particular, HST at 40 and 80 μM significantly reduces MDI-induced adipogenesis in 3T3-L1 preadipocytes[1].
Hirsutenone (0-100 μM; 48 hours) reduces the protein expression levels of PPARg, C/EBPa, and FAS in a dose-dependent manner in 3T3-L1 preadipocytes[1].
Hirsutenone (80 μM; 20-24 hours) suppresses the cell cycle entry to S and G2/M phases occurrs at 20 hours when compares with at 20 hours. At 24 h, more cells are arrested in G1 phase (53% of total cells) when compares with the MDI-induced group which contains the majority of cells (56% of total cells) in G2/M phase[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hirsutenone Related Antibodies
Molecular Weight

328.36

Formula

C19H20O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=C(O)C=CC(CC/C=C/C(CCC2=CC(O)=C(O)C=C2)=O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
References
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Hirsutenone Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hirsutenone41137-87-5PI3KERKPhosphoinositide 3-kinaseExtracellular signal regulated kinasesC/EBPaHSTMCEmitoticclonalexpansionanti-inflammatoryanti-tumoranti-adipogenicdifferentiation3T3-L1preadipocyteInhibitorinhibitorinhibit

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