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  4. HKOCl-4

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.

For research use only. We do not sell to patients.

HKOCl-4 Chemical Structure

HKOCl-4 Chemical Structure

CAS No. : 2031170-85-9

Size Price Stock Quantity
1 mg USD 375 In-stock
5 mg USD 990 In-stock
10 mg USD 1628 In-stock
25 mg USD 3256 In-stock
50 mg USD 4884 In-stock
100 mg USD 7326 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

HKOCl-4 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE HKOCl-4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity[1]. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.

IC50 & Target

Ex: 530 nm; Em 557 nm
In Vitro

HKOCl-4 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].
HKOCl-4 exhibits a ultra-fast response towards 1 equiv of HOCl (reached a plateau within 2 min) , and it shows a >20-fold increase in fluorescence intensity over other analytes (1 equiv of OH and ONOO as well as 10 equiv of O2, NO, 1O2, ROO , TBHP and H2O2)[2].
HKOCl-4 is first tested and it was proven to be non-toxic in living cells even at a concentration up to 50 μM in RAW264.7 macrophages[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKOCl-4 Related Antibodies
Molecular Weight

688.59

Formula

C38H35Cl2NO7
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

527
Excitation (Ex)

490
SMILES

O=C(OCC)CCCN1C(C)(C)CC(C)C2=C1C=C3C(C4(OC(C5=C4C=CC=C5)=O)C6=CC=C(OC7=CC(Cl)=C(O)C(Cl)=C7)C=C6O3)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (290.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4522 mL 7.2612 mL 14.5224 mL
5 mM 0.2904 mL 1.4522 mL 2.9045 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 5 mg/mL (7.26 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4522 mL 7.2612 mL 14.5224 mL 36.3061 mL
5 mM 0.2904 mL 1.4522 mL 2.9045 mL 7.2612 mL
10 mM 0.1452 mL 0.7261 mL 1.4522 mL 3.6306 mL
15 mM 0.0968 mL 0.4841 mL 0.9682 mL 2.4204 mL
20 mM 0.0726 mL 0.3631 mL 0.7261 mL 1.8153 mL
25 mM 0.0581 mL 0.2904 mL 0.5809 mL 1.4522 mL
30 mM 0.0484 mL 0.2420 mL 0.4841 mL 1.2102 mL
40 mM 0.0363 mL 0.1815 mL 0.3631 mL 0.9077 mL
50 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7261 mL
60 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6051 mL
80 mM 0.0182 mL 0.0908 mL 0.1815 mL 0.4538 mL
100 mM 0.0145 mL 0.0726 mL 0.1452 mL 0.3631 mL
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HKOCl-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKOCl-42031170-85-9BXY2142HKOCl4HKOCl 4BXY 2142BXY-2142Fluorescent DyeHOClrhodol-basedFluorescent Probehypochlorous acidO2NO1O2ROO?TBHPInhibitorinhibitorinhibit

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