2. Others Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Fluorescent Dye Reactive Oxygen Species
  4. Hydroxyphenyl Fluorescein

Hydroxyphenyl Fluorescein  (Synonyms: HPF; 3'-p-(Hydroxyphenyl) fluorescein)

Cat. No.: HY-111330 Purity: 99.91%
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Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm.

For research use only. We do not sell to patients.

Hydroxyphenyl Fluorescein Chemical Structure

Hydroxyphenyl Fluorescein Chemical Structure

CAS No. : 359010-69-8

Size Price Stock Quantity
1 mg USD 350 In-stock
5 mg USD 990 In-stock
10 mg USD 1485 In-stock
25 mg USD 2550 In-stock
50 mg USD 3570 In-stock
100 mg USD 4998 In-stock
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Based on 2 publication(s) in Google Scholar

Hydroxyphenyl Fluorescein Related Antibodies

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Description

Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm[1].

In Vitro

Preparation of HPF working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of HPF to obtain 10 mM of HPF.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HPF working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of HPF working solution.
Note: Please adjust the concentration of HPF working solution according to the actual situation.

Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of HPF working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hydroxyphenyl Fluorescein Related Antibodies
In Vivo

The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of OH by the fluorescence signal emitted by the oxidized form of HPF is assessed[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

424.40

Formula

C26H16O6
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

515
Excitation (Ex)

490
SMILES

OC1=CC=C(OC2=CC3=C(C4(C5=CC=C(C=C5O3)O)C6=C(C(O4)=O)C=CC=C6)C=C2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (294.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3563 mL 11.7813 mL 23.5627 mL
5 mM 0.4713 mL 2.3563 mL 4.7125 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3563 mL 11.7813 mL 23.5627 mL 58.9067 mL
5 mM 0.4713 mL 2.3563 mL 4.7125 mL 11.7813 mL
10 mM 0.2356 mL 1.1781 mL 2.3563 mL 5.8907 mL
15 mM 0.1571 mL 0.7854 mL 1.5708 mL 3.9271 mL
20 mM 0.1178 mL 0.5891 mL 1.1781 mL 2.9453 mL
25 mM 0.0943 mL 0.4713 mL 0.9425 mL 2.3563 mL
30 mM 0.0785 mL 0.3927 mL 0.7854 mL 1.9636 mL
40 mM 0.0589 mL 0.2945 mL 0.5891 mL 1.4727 mL
50 mM 0.0471 mL 0.2356 mL 0.4713 mL 1.1781 mL
60 mM 0.0393 mL 0.1964 mL 0.3927 mL 0.9818 mL
80 mM 0.0295 mL 0.1473 mL 0.2945 mL 0.7363 mL
100 mM 0.0236 mL 0.1178 mL 0.2356 mL 0.5891 mL
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Hydroxyphenyl Fluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hydroxyphenyl Fluorescein359010-69-8HPF 3'-p-(Hydroxyphenyl) fluoresceinFluorescent DyeReactive Oxygen SpeciesROSprobeOHindicatorfluoresceperoxynitriteInhibitorinhibitorinhibit

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