I2959

Cat. No.: HY-W013508 Purity: 98.11%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

I2959 is widely used as a photoinitiator for photochemical cross-linking of hydrogels, and the combination of I2959 and CMA hydrogel has been used as a bio-ink for biological 3D printing applications. In addition, I2959 has a high free radical generation efficiency and is cytotoxic to rapidly dividing cell lines.

For research use only. We do not sell to patients.

I2959 Chemical Structure

CAS No. : 106797-53-9

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 g	USD 30	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Method for encapsulation of Saos-2 cells in photochemically cross-linked CMA hydrogels^[1]:
1. Human osteosarcoma cell line (Saos-2 cells) are propagated in RPMI medium containing 15% FBS, 1% L-glutamine, and 1% penicillin/streptomycin.
2. Cells are suspended in neutralized CMA solution (containing I2959 at 0.02% or 0.1% concentration).
3. 45 µL of cell suspension is added to each well of a 96-well plate and incubated at 37°C for 30 min for gelation (5000 cells/gel).
4. Gels are exposed to UV light (365 nm) for two different times (1 min or 10 min) for photocross-linking. Hydrogels not exposed to UV light were used as controls for cell studies.
5. Culture the cell-encapsulated hydrogels in α-MEM medium containing 50 µg/mL ascorbic acid, 10 mM β-glycerophosphate, 10% FBS, and 1% penicillin/streptomycin for up to 14 days.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

224.26

Formula

C₁₂H₁₆O₄

CAS No.

106797-53-9

Appearance

Solid

Color

White to yellow

SMILES

CC(C)(O)C(=O)C1=CC=C(OCCO)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (445.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4591 mL	22.2956 mL	44.5911 mL
5 mM	0.8918 mL	4.4591 mL	8.9182 mL
10 mM	0.4459 mL	2.2296 mL	4.4591 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.67%

Select Batch:

Data Sheet (269 KB) SDS (458 KB)

COA (244 KB) HNMR (117 KB) RP-HPLC (228 KB)

Handling Instructions (2659 KB)

References

[1]. Thuy-Uyen Nguyen, et al. Photochemically crosslinked cell-laden methacrylated collagen hydrogels with high cell viability and functionality. J Biomed Mater Res A. 2019 Jul;107(7):1541-1550. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4591 mL	22.2956 mL	44.5911 mL	111.4778 mL
	5 mM	0.8918 mL	4.4591 mL	8.9182 mL	22.2956 mL
	10 mM	0.4459 mL	2.2296 mL	4.4591 mL	11.1478 mL
	15 mM	0.2973 mL	1.4864 mL	2.9727 mL	7.4319 mL
	20 mM	0.2230 mL	1.1148 mL	2.2296 mL	5.5739 mL
	25 mM	0.1784 mL	0.8918 mL	1.7836 mL	4.4591 mL
	30 mM	0.1486 mL	0.7432 mL	1.4864 mL	3.7159 mL
	40 mM	0.1115 mL	0.5574 mL	1.1148 mL	2.7869 mL
	50 mM	0.0892 mL	0.4459 mL	0.8918 mL	2.2296 mL
	60 mM	0.0743 mL	0.3716 mL	0.7432 mL	1.8580 mL
	80 mM	0.0557 mL	0.2787 mL	0.5574 mL	1.3935 mL
	100 mM	0.0446 mL	0.2230 mL	0.4459 mL	1.1148 mL

I2959 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

I2959106797-53-9I 2959I-2959OthersPhotoinitiatorphotochemical crosslinkinghydrogelsradical generationcytotoxicityInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks