2. Metabolic Enzyme/Protease
  3. Indoleamine 2,3-Dioxygenase (IDO)
  4. IDO2-IN-1

IDO2-IN-1 is an orally active and potent Indoleamine 2,3-dioxygenase 2 (IDO2) inhibitor with an IC50 value of 112 nM. IDO2-IN-1 can be used for inflammatory autoimmunity research.

For research use only. We do not sell to patients.

IDO2-IN-1 Chemical Structure

IDO2-IN-1 Chemical Structure

CAS No. : 2803768-09-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 572 In-stock
Solution
10 mM * 1 mL in DMSO USD 572 In-stock
Solid
1 mg USD 192 In-stock
5 mg USD 480 In-stock
10 mg USD 760 In-stock
25 mg USD 1520 In-stock
50 mg USD 2430 In-stock
100 mg USD 3880 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

IDO2-IN-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE IDO2-IN-1

View All Indoleamine 2,3-Dioxygenase (IDO) Isoform Specific Products:

View All Isoforms
IDO1 IDO2 IDO

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  • Purity & Documentation

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Description

IDO2-IN-1 is an orally active and potent Indoleamine 2,3-dioxygenase 2 (IDO2) inhibitor with an IC50 value of 112 nM. IDO2-IN-1 can be used for inflammatory autoimmunity research[1].

IC50 & Target

IDO1

411 nM (IC50)

IDO2

112 (IC50)

In Vitro

IDO2-IN-1 (compound 22) shows stronger inhibition on IDO2 (IC50 =112 nM) over IDO1 (IC50 =411 nM)[1].
IDO2-IN-1 inhibits hIDO1 expression (EC50=633 nM) in HeLa cell-based IDO1/kynurenine assay, co-incubated with hIFN-γ (100 ng/mL final concentration), which is used for producing N-formylkynurenine[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IDO2-IN-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: HeLa cells line expressing hIDO1 induced by IFN-γ
Concentration: 1 nM-0.1 mM
Incubation Time: 48 hours
Result: Showed additional potency against IDO1 with an EC50 value of 633 nM.
In Vivo

The Adjuvant arthritis (AA) model and Collagen-induced arthritis (CIA) model have similar pathogenesis and pathological characteristics to human rheumatoid arthritis (RA).
IDO2-IN-1 (compound 22) (100 mg/kg; p.o.; once dose) exhibits excellent anti-inflammatory activity, higher than naproxen, a prescription drug reducing pain, swelling, and joint stiffness from arthritis[1].
IDO2-IN-1 (25, 50, 100 mg/kg; i.p.; once daily, for 19 d) exhibits excellent inhibitory effect on mice paw swelling, shows efficacy in a collagen-induced arthritis model in mice[1].
IDO2-IN-1 (30, 60, 120 mg/kg; i.p.; once daily, for 15 d) inhibits joint inflammation and displays potential effect in autoimmune arthritis improvement[1].
Pharmacokinetic Profile in Rat[1]

Route Dose (mg/kg) T1/2/sub> (h) Tmax (h) Cmax (ng/mL) AUC(0-∞) (h•ng/mL) CL (mL/h/kg) Vz (mL/kg) MRT(0-∞) (h) F (%)
i.v. 1 0.69 / / 375.1 2673 2675 0.55 /
p.o. 10 2.02 0.75 153.8 670.5 / / 7.48 17.87

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Xylene-induced ear swelling mouse model (ICR mice, male, 6 weeks old)[1]
Dosage: 100 mg/kg
Administration: Oral gavage; once dose; evenly coated right ear with 25 μL of xylene after 1 h treatment
Result: Significantly relieved mouse ear swelling with a high swelling inhibition rate of 65.32%.
Animal Model: Collagen-induced arthritis (CIA) mice model (DBA/1J mice, male, 6 weeks old)[1]
Dosage: 25, 50, 100 mg/kg
Administration: Intraperitoneal injection; once daily; 19 days, began on day 56 after collagen induced
Result: Decreased the expression of inflammatory cytokines IL-18 and IL-33.
Reduced inflammation and cartilage and bone erosions symptoms.
Animal Model: Adjuvant arthritis (AA) rat model (Sprague-Dawley rats, male, 180 ± 20 g)[1]
Dosage: 30, 60, 120 mg/kg
Administration: Intraperitoneal injection; once daily; 15 days, began on day 21 after chondrex induced
Result: Significantly reduced IL-6 and TNF-α levels.
Decreased synovial hyperplasia accompanied by inflammatory cell infiltration, pannus formation, and bone erosion of cartilage in a dose-dependent manner.
Molecular Weight

541.36

Formula

C21H21BrN10O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

BrC1=CC=CC(CN/C(C2=NON=C2NCCN3C=C(N=N3)CNC(C4=NC=CC=C4)=O)=N\O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 140 mg/mL (258.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8472 mL 9.2360 mL 18.4720 mL
5 mM 0.3694 mL 1.8472 mL 3.6944 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8472 mL 9.2360 mL 18.4720 mL 46.1800 mL
5 mM 0.3694 mL 1.8472 mL 3.6944 mL 9.2360 mL
10 mM 0.1847 mL 0.9236 mL 1.8472 mL 4.6180 mL
15 mM 0.1231 mL 0.6157 mL 1.2315 mL 3.0787 mL
20 mM 0.0924 mL 0.4618 mL 0.9236 mL 2.3090 mL
25 mM 0.0739 mL 0.3694 mL 0.7389 mL 1.8472 mL
30 mM 0.0616 mL 0.3079 mL 0.6157 mL 1.5393 mL
40 mM 0.0462 mL 0.2309 mL 0.4618 mL 1.1545 mL
50 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9236 mL
60 mM 0.0308 mL 0.1539 mL 0.3079 mL 0.7697 mL
80 mM 0.0231 mL 0.1154 mL 0.2309 mL 0.5772 mL
100 mM 0.0185 mL 0.0924 mL 0.1847 mL 0.4618 mL
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IDO2-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IDO2-IN-12803768-09-2Indoleamine 2,3-Dioxygenase (IDO)immunotherapeutic avenuesrheumatoid arthritisindoleamine 2,3-dioxygenase 2autoimmune diseasesimmunomodulatory enzymeinflammatory autoimmunitycollagen-induced arthritis mice modeladjuvant arthritis rat modelpathogenic mediatorcancer immunologyneurodegenerative diseasesand neurologicalInhibitorinhibitorinhibit

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