2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Apoptosis and Necrosis
  6. Annexin V-iFluor 488/PI Apoptosis Detection Kit

Annexin V-iFluor 488/PI Apoptosis Detection Kit 

Cat. No.: HY-K1080
Manual SDS

MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-),, early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).

Annexin V-iFluor 488/PI Apoptosis Detection Kit
Size Price Stock Quantity
Free Sample (10T)   Apply Now  
25T USD 96 In-stock
50T USD 167 In-stock
100T USD 285 In-stock
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1 Publications Citing Use of MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS translocates from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with iFluor 488 can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium Iodide (PI) is a cell-membrane impermeable dye to live cells and early apoptosis cells, but stains late apoptosis cells and necrosis cells with red fluorescence. After staining cells with Annexin V-iFluor 488 and PI, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).
Storage

4°C, 2 years.

Protect from light.

Components
Components HY-K1080-25T HY-K1080-50T HY-K1080-100T
Annexin V-iFluor 488 125 μL 250 μL 500 μL
10× Binding Buffer 2 mL 2 × 2 mL 3 × 2 mL
PI Solution 125 μL 250 μL 500 μL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Annexin V-iFluor 488/PI Apoptosis Detection Kit
Cat. No.:
HY-K1080
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