2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Apoptosis and Necrosis
  6. Cell Cycle and Apoptosis Analysis Kit (PI staining)

Cell Cycle and Apoptosis Analysis Kit (PI staining) 

Cat. No.: HY-K1071
Manual SDS

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.


Cell Cycle and Apoptosis Analysis Kit (PI staining)
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50T USD 65 In-stock
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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis. The content of DNA is changed with the process of cell cycle. DNA can be stained by fluorescent dye, such as Propidium Iodide (PI), to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1/S/G2/M phase as well as apoptosis cells with signals at sub-G1 region. PI is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. However, PI also binds to RNA, RNase A provided in this kit can eliminate RNA interference. In addition, light scattering also changes during apoptosis. In early apoptosis, light signals from Forward Scatter (FSC) become weak and signals from Side Scatter (SSC) increase or remain unchanged. When cells proceed to the phase of late apoptosis, signals from FSC/SSC both decrease.


Storage

4℃, 6 months; -20℃,1 year.


Protect from light.
Components
Components HY-K1071-50T
Cell Stain Buffer 25 mL
PI Stain (20×) 1.25 mL
RNase A (50×) 0.5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cell Cycle and Apoptosis Analysis Kit (PI staining)
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HY-K1071
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