Mycoplasma Detection Kit (Luminescence) 

Mycoplasma are small, structurally simple bacteria which lack a cell wall and represent one of the most prevalent and serious sources of cell line contamination. Mycoplasma contamination is invisible, as it causes no discernible change in turbidity or pH even at densities as high as 10⁸ cells/mL, making it particularly insidious. Mycoplasma infections are highly toxic and can induce a range of changes in cell cultures, including reduced growth rates, abnormal morphology, chromosomal abnormalities, and metabolic disturbances. These changes can lead to slowed cell growth, aberrant differentiation, or even cell death, severely disrupting normal cell functions. Mycoplasma contamination not only significantly affects the reliability, repeatability, and consistency of experimental results but can also lead to experimental failure. Therefore, timely and accurate detection of mycoplasma contamination is crucial for ensuring the quality of cell cultures and the accuracy of experimental outcomes.

MCE Mycoplasma Detection Kit (Luminescence) utilizes chemiluminescence technology to detect the activity of specific enzymes in the sample, determining the presence of mycoplasma contamination. The detection principle is as follows: under the action of luciferase, ATP reacts with luciferin to produce fluorescence. Specific enzymes released by mycoplasma can catalyze the conversion of ADP to ATP. The generated ATP further reacts with luciferase, enhancing the fluorescence signal. By detecting changes in bioluminescent signals, the presence of mycoplasma contamination in the sample can be determined.

Features of MCE Mycoplasma Detection Kit (Luminescence)

1. High Sensitivity: Detection sensitivity is < 25 CFU/mL.

2. Rapid: The detection process can be completed within 30 min.

3. Accurate: Detects biologically active mycoplasma, providing more accurate results compared to the PCR assay.

-20℃, 1 years

Keep away from light and avoid repeated freeze-thaw cycles