Mycoplasma Detection Kit (qPCR) 

Mycoplasma are small, structurally simple bacteria which lack a cell wall and represent one of the most prevalent and serious sources of cell line contamination. Mycoplasma contamination is invisible, as it causes no discernible change in turbidity or pH even at densities as high as 10⁸ cells/mL, making it particularly insidious. Mycoplasma infections are highly toxic and can induce a range of changes in cell cultures, including reduced growth rates, abnormal morphology, chromosomal abnormalities, and metabolic disturbances. These changes can lead to slowed cell growth, aberrant differentiation, or even cell death, severely disrupting normal cell functions. Mycoplasma contamination not only significantly affects the reliability, repeatability, and consistency of experimental results but can also lead to experimental failure. Therefore, timely and accurate detection of mycoplasma contamination is crucial for ensuring the quality of cell cultures and the accuracy of experimental outcomes.

MCE Mycoplasma Detection Kit (qPCR) is a diagnostic kit that utilizes the real-time quantitative PCR (qPCR) method to detect mycoplasma contamination in biological materials such as cultured cells. The kit includes qPCR premix and specific primer-probe targeting the conserved regions of the mycoplasma, enabling the specific amplification and detection of mycoplasma DNA.

Features of MCE Mycoplasma Detection Kit (PCR)

1.  Optimized Premix Formula: The specially optimized reaction buffer introduces dUTP/UDG anti-contamination systems, which can degrade U-containing ssDNA and dsDNA before reverse transcription, effectively preventing cross-contamination caused by PCR products.

2.  High Sensitivity: For extracted mycoplasma DNA in samples, the detection limit reaches as low as 10 CFU/mL.

3.  Specific Fluorescent Probes: Utilizes FAM and VIC fluorescent probes for the specific amplification of mycoplasma and internal controls, respectively.

4.  Broad detection range: Capable of detecting a wide range of species under the class Mollicutes, including various Mycoplasma spp., Acholeplasmaspp. and Spiroplasmaspp.), meeting the requirements of nucleic acid amplification techniques (NAT) in mycoplasma detection in EP 2.6.7 and JP G3.

-20℃, 2 years

Keep away from light and avoid repeated freeze-thaw cycles.