SYBR Green qPCR Master Mix (Low ROX) 

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer and ddH₂O.

MCE qPCR Master Mix (Low ROX) can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Low ROX Reference Dye is included for use with instruments that require a low level of reference dye for rectify the error of fluorescence signals between different wells.

MCE qPCR Master Mix (Low ROX) is applied for the Real-Time PCR instrument as follows:

ABI: 7500 (Fast), ViiA 7, QuantStudio 6 and 7 Flex Systems; Stratagene: Mx3000P, Mx3005P and Mx4000; Qiagen/Corbett Rotor-Gene: 3000; Bio-Rad/MJ: Chromo4, Opticon 2 and Opticon

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Low ROX Reference Dye is included.

Stored at -20°C protecting from light, and is stable for up to 24 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.