2. MCE Kits
  3. Molecular Biology
  4. PCR & qPCR
  5. Real-Time PCR
  6. SYBR Green qPCR Master Mix

SYBR Green qPCR Master Mix 

Cat. No.: HY-K0501
Manual SDS

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.

SYBR Green qPCR Master Mix
Size Price Stock Quantity
Free Sample (50 rxns)   Apply Now  
100 rxns USD 40 In-stock
500 rxns USD 132 In-stock
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201 Publications Citing Use of MCE SYBR Green qPCR Master Mix

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells.

 

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Passive reference dyes are provided for different qPCR instruments.
Storage

-20°C, 2 years

Protecting from light, and avoid repetitive freeze-thaw cycles
Components
Components HY-K0501-100rxns HY-K0501-500rxns
SYBR Green qPCR Master Mix (2×) 1 mL 1 mL × 5
ROX Reference Dye (50×) 40 μL 40 μL × 5
ROX Reference Dye Ⅱ (50×) 40 μL 40 μL × 5
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SYBR Green qPCR Master Mix
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HY-K0501
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