SYBR Green qPCR Master Mix (No ROX) 

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer and ddH₂O.

MCE qPCR Master Mix (No ROX) can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. No ROX Reference Dye is included for use with instruments that does not require a reference dye for rectify the error of fluorescence signals between different wells.

MCE qPCR Master Mix (No ROX) is applied for the Real-Time PCR instrument as follows:

Bio-Rad: CFX96, CFX384, iCycler IQ, MyiQ, iQ5, MiniOpticon, Opticon, Opticon 2 and 4;

Eppendorf: Mastercycler ep realplex, realplex2 s;

Qiagen: Corbett Rotor-Gene Q, Corbett Rotor-Gene 3000, Corbett Rotor-Gene 6000;

Roche: LightCycler 480, LightCycler 2.0; LightCycler 96;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. No ROX Reference Dye is included.

Stored at -20°C protecting from light, and is stable for up to 24 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.