iNOS/COX-2-IN-1 

iNOS/COX-2-IN-1 (Compound 12e) is an inhibitor of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). iNOS/COX-2-IN-1 inhibits the NF-κB and MAPKs signaling pathways and thus exerts anti-inflammatory effects^[1].

iNOS/COX-2-IN-1 (0.625-40 μM; 24 h) has no significant cytotoxicity against RAW 264.7 cells at a concentration of 5 μM^[1].
iNOS/COX-2-IN-1 (2.5-10 μM; 1 h) significantly inhibits LPS-induced NO production, with a NO inhibition rate of 45.38% (5 μM). Concentration-dependently inhibits the expression of TNF-α and COX-2. Effectively inhibits mRNA expression of pro-inflammatory cytokines. Exerts its anti-inflammatory effect by inhibiting the activation of NF-κB signaling pathway ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

535.72

C₃₁H₃₈FN₃O₂S

FC1=CC=CC(C2CC([C@H]3CC[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@@]43C)=NN2C(SC7)=NC7=O)=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Cai X, et al. Design, synthesis and molecular modeling of novel D-ring substituted steroidal 4,5-dihydropyrazole thiazolinone derivatives as anti-inflammatory agents by inhibition of COX-2/iNOS production and down-regulation of NF-κB/MAPKs in LPS-induced RAW264.7 macrophage cells. Eur J Med Chem. 2024 Apr 27;272:116460.  [Content Brief]