2. Peptides
  3. Peptide and Derivatives
  4. Inhibitors and Substrates
  5. JAG-1, scrambled

JAG-1, scrambled (scJag-1) is a scrambled sequence of JAG-1 (Jagged-1 protein). JAG-1, scrambled has a random sequence of the amino acids that are the same as the active fragment. JAG-1, scrambled is usually used as a negative control.

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JAG-1, scrambled Chemical Structure

JAG-1, scrambled Chemical Structure

CAS No. : 402941-23-5

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Other Forms of JAG-1, scrambled:

JAG-1, scrambled Related Antibodies

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Description

JAG-1, scrambled (scJag-1) is a scrambled sequence of JAG-1 (Jagged-1 protein). JAG-1, scrambled has a random sequence of the amino acids that are the same as the active fragment. JAG-1, scrambled is usually used as a negative control[1].

In Vitro

In contrast to JAG-1, pretreatment of human pulmonary arterial smooth muscle cells (PASMC) with 50 μM scJag-1 for 30 min has no effect on the transient and plateau components of store-operated Ca2+ entry (SOCE) in comparison to the cells treated with vehicle used to dissolve scJag-1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JAG-1, scrambled Related Antibodies
Molecular Weight

2107.35

Formula

C93H127N25O26S3
CAS No.
Sequence

Arg-Cys-Gly-Pro-Asp-Cys-Phe-Asp-Asn-Tyr-Gly-Arg-Tyr-Lys-Tyr-Cys-Phe
Sequence Shortening

RCGPDCFDNYGRYKYCF
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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JAG-1, scrambled Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JAG-1, scrambled402941-23-5scJag-1scJag1scJag 1OthersnegativecontrolscrambledsequencerandominactiveInhibitorinhibitorinhibit

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JAG-1, scrambled
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HY-P1849
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