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  3. Fluorescent Dye
  4. Janelia Fluor® 526, SE

Janelia Fluor® 526, SE  (Synonyms: JF526, SE; JF526, NHS)

Cat. No.: HY-138658
Handling Instructions

Molarity Calculator

Janelia Fluor 526, SE (JF526,SE) is a fluorogenic yellow fluorescent dye that contains NHS ester group. JF526 is a versatile scaffold for fluorogenic ligands, including labels for genetically encoded self-labeling protein tags and stains for endogenous structures. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 526, SE Chemical Structure

Janelia Fluor® 526, SE Chemical Structure

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Janelia Fluor® 526, SE Related Antibodies

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Description

Janelia Fluor 526, SE (JF526,SE) is a fluorogenic yellow fluorescent dye that contains NHS ester group. JF526 is a versatile scaffold for fluorogenic ligands, including labels for genetically encoded self-labeling protein tags and stains for endogenous structures[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

Excitation maximum=526 nm; emission maximum=550 nm[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Janelia Fluor® 526, SE Related Antibodies
Molecular Weight

659.49

Formula

C31H19F6N3O7
Emission (Em)

526
Excitation (Ex)

488
SMILES

FC(F)(C/1)C[N+]1=C2C(F)=CC3=C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC(F)=C(N7CC(F)(C7)F)C=C6OC3=C\2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Janelia Fluor® 526, SE Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 526, SEJF526, SE JF526, NHSFluorescent DyeInhibitorinhibitorinhibit

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