Janelia Fluor® 585, SE  (Synonyms: JF585, SE; JF585, NHS)

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments^[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

Maximum absorption wavelength (λabs)=585 nm, maximum emission wavelength (λem)=609 nm^[1].
The JF585-SNAP-tag ligand (24) also functioned as a cellular label. The new HaloTag ligands derived from JF585 and JF635 show a high degree of chromogenicity and fluorogenicity, a critical parameter in advanced imaging experiments^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

649.59

C₃₄H₂₇F₄N₃O₆

1811539-88-4

585

565

FC(C/1)(F)C[N+]1=C2C=C3C(C)(C)C4=C(C=CC(N5CC(F)(F)C5)=C4)C(C6=CC(C(ON7C(CCC7=O)=O)=O)=CC=C6C([O-])=O)=C3C=C/2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403  [Content Brief]