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  4. Janelia Fluor® 635, SE

Janelia Fluor® 635, SE  (Synonyms: JF635, SE; JF635, NHS)

Cat. No.: HY-131026 Purity: 99.30%
SDS COA Handling Instructions

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Janelia Fluor® 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 635, SE Chemical Structure

Janelia Fluor® 635, SE Chemical Structure

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1 mg USD 644 In-stock
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Based on 1 publication(s) in Google Scholar

Janelia Fluor® 635, SE Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

Janelia Fluor® 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

Maximum absorption wavelength (λabs)=635 nm, maximum emission wavelength (λem)=652 nm[1].
JF635 can serve as a ligand for self-labeling tag (such as HaloTag and SNAP-tag). The SNAP-tag ligand of JF635 (29) effectively labels SNAP-tag fusions in cells and the JF635–HaloTag ligand (27) could be used in a two-color experiment with JF525-SNAP-tag ligand[1],

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Janelia Fluor® 635, SE Related Antibodies
Molecular Weight

629.68

Formula

C33H29F2N3O6Si
Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CC(C4)F)=C(C3=C/5)C=CC5=[N+]6CC(C/6)F)=C1)C([O-])=O)ON7C(CCC7=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 62.96 mg/mL (99.99 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5881 mL 7.9405 mL 15.8811 mL
5 mM 0.3176 mL 1.5881 mL 3.1762 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.30%

SDS (252 KB)

COA (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5881 mL 7.9405 mL 15.8811 mL 39.7027 mL
5 mM 0.3176 mL 1.5881 mL 3.1762 mL 7.9405 mL
10 mM 0.1588 mL 0.7941 mL 1.5881 mL 3.9703 mL
15 mM 0.1059 mL 0.5294 mL 1.0587 mL 2.6468 mL
20 mM 0.0794 mL 0.3970 mL 0.7941 mL 1.9851 mL
25 mM 0.0635 mL 0.3176 mL 0.6352 mL 1.5881 mL
30 mM 0.0529 mL 0.2647 mL 0.5294 mL 1.3234 mL
40 mM 0.0397 mL 0.1985 mL 0.3970 mL 0.9926 mL
50 mM 0.0318 mL 0.1588 mL 0.3176 mL 0.7941 mL
60 mM 0.0265 mL 0.1323 mL 0.2647 mL 0.6617 mL
80 mM 0.0199 mL 0.0993 mL 0.1985 mL 0.4963 mL
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Janelia Fluor® 635, SE Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 635, SEJF635, SE JF635, NHSFluorescent DyeredfluorescentdyelivecellimagingSRMInhibitorinhibitorinhibit

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