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  3. Fluorescent Dye
  4. Janelia Fluor® 635, SE

Janelia Fluor® 635, SE  (Synonyms: JF635, SE; JF635, NHS)

Cat. No.: HY-131026
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Janelia Fluor® 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 635, SE Chemical Structure

Janelia Fluor® 635, SE Chemical Structure

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Based on 1 publication(s) in Google Scholar

Janelia Fluor® 635, SE Related Antibodies

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Description

Janelia Fluor® 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

Maximum absorption wavelength (λabs)=635 nm, maximum emission wavelength (λem)=652 nm[1].
JF635 can serve as a ligand for self-labeling tag (such as HaloTag and SNAP-tag). The SNAP-tag ligand of JF635 (29) effectively labels SNAP-tag fusions in cells and the JF635–HaloTag ligand (27) could be used in a two-color experiment with JF525-SNAP-tag ligand[1],

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Janelia Fluor® 635, SE Related Antibodies
Molecular Weight

629.68

Formula

C33H29F2N3O6Si
SMILES

O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CC(C4)F)=C(C3=C/5)C=CC5=[N+]6CC(C/6)F)=C1)C([O-])=O)ON7C(CCC7=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Janelia Fluor® 635, SE Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 635, SEJF635, SE JF635, NHSFluorescent DyeredfluorescentdyelivecellimagingSRMInhibitorinhibitorinhibit

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