2. Epigenetics
  3. Protein Arginine Deiminase
  4. JBI-589

JBI-589 is a non-covalent PAD4 isoform-selective inhibitor with oral bioavailability. JBI-589 reduces CXCR2 expression and blocks neutrophil chemotaxis. JBI-589 reduces primary tumor and metastases, and enhances the anti-tumor effect of checkpoint inhibitors. JBI-589 can be used in cancer research.

For research use only. We do not sell to patients.

JBI-589 Chemical Structure

JBI-589 Chemical Structure

CAS No. : 2308504-22-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 1589 In-stock
Solution
10 mM * 1 mL in DMSO USD 1589 In-stock
Solid
1 mg USD 500 In-stock
5 mg USD 1500 In-stock
10 mg USD 2600 In-stock
25 mg USD 5800 In-stock
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Based on 1 publication(s) in Google Scholar

JBI-589 Related Antibodies

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Description

JBI-589 is a non-covalent PAD4 isoform-selective inhibitor with oral bioavailability. JBI-589 reduces CXCR2 expression and blocks neutrophil chemotaxis. JBI-589 reduces primary tumor and metastases, and enhances the anti-tumor effect of checkpoint inhibitors. JBI-589 can be used in cancer research[1][2].

In Vitro

JBI-589 (1 μM, 2 h) significantly downregulates the expression of CXCR2 on neutrophils and reduces their migration induced by CXCL1 in vitro, with no effect on the suppressive activity of Ly6G cells generated from HPCs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JBI-589 Related Antibodies

Western Blot Analysis[1]

Cell Line: Phorbol 12-myristate 13-acetate (HY-18739)-induced Mouse neutrophils
Concentration: 0.25-1 μM
Incubation Time: 4 h
Result: Inhibited the citrullination of histone H3 in Phorbol 12-myristate 13-acetate (HY-18739)-induced mouse neutrophils in a dose-dependent manner, with no apparent toxicity.
In Vivo

JBI-589 (50 mg/kg, p.o., twice a day for 24 days) significantly inhibits the growth of primary tumors in LL2 and B16F10 tumor C57BL/6 mouse models[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: LL2 tumor C57BL/6 mouse model; B16F10 tumor C57BL/6 mouse model
Dosage: 50 mg/kg
Administration: Oral gavage (p.o.), twice daily
Result: Led to a significant downregulation of CXCR2 receptor expression in Ly6G cells of the mice, while CXCR4 receptor expression remained unchanged.
Had no effect on the cell counts of bone marrow cells, CD8 T cells, and NK cells in tumor-bearing mice model.
Molecular Weight

481.56

Formula

C29H28FN5O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(N1C(C=C(C=C1)C(N2C[C@@H](CCC2)N)=O)=N3)=C3C4=CC5=CC=CC=C5N4CC6=CC=C(C=C6)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (259.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0766 mL 10.3829 mL 20.7658 mL
5 mM 0.4153 mL 2.0766 mL 4.1532 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.93%

COA (250 KB) HNMR (144 KB) LCMS (97 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0766 mL 10.3829 mL 20.7658 mL 51.9146 mL
5 mM 0.4153 mL 2.0766 mL 4.1532 mL 10.3829 mL
10 mM 0.2077 mL 1.0383 mL 2.0766 mL 5.1915 mL
15 mM 0.1384 mL 0.6922 mL 1.3844 mL 3.4610 mL
20 mM 0.1038 mL 0.5191 mL 1.0383 mL 2.5957 mL
25 mM 0.0831 mL 0.4153 mL 0.8306 mL 2.0766 mL
30 mM 0.0692 mL 0.3461 mL 0.6922 mL 1.7305 mL
40 mM 0.0519 mL 0.2596 mL 0.5191 mL 1.2979 mL
50 mM 0.0415 mL 0.2077 mL 0.4153 mL 1.0383 mL
60 mM 0.0346 mL 0.1730 mL 0.3461 mL 0.8652 mL
80 mM 0.0260 mL 0.1298 mL 0.2596 mL 0.6489 mL
100 mM 0.0208 mL 0.1038 mL 0.2077 mL 0.5191 mL
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JBI-589 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JBI-5892308504-22-3JBI589JBI 589Protein Arginine DeiminasePeptidylarginine DeiminaseAnti-tumorprimary tumormetastasescheckpointInhibitorinhibitorinhibit

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