2. Others Metabolic Enzyme/Protease
  3. Others Ser/Thr Protease
  4. LM2I

LM2I is a derivative of Spinosyn A (SPA). LM2I is argininosuccinate synthase (ASS1) enzyme activator, and tumor inhibitor that directly interact with ASS1. LM2I has significant antiproliferative activity in seven colorectal cancer cell-lines and xenograft tumors of colorectal cancer. LM2I inhibits colorectal cancer cell growth via the EGFR pathway.

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LM2I Chemical Structure

LM2I Chemical Structure

CAS No. : 2055494-50-1

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Based on 1 publication(s) in Google Scholar

LM2I Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

LM2I is a derivative of Spinosyn A (SPA). LM2I is argininosuccinate synthase (ASS1) enzyme activator, and tumor inhibitor that directly interact with ASS1. LM2I has significant antiproliferative activity in seven colorectal cancer cell-lines and xenograft tumors of colorectal cancer. LM2I inhibits colorectal cancer cell growth via the EGFR pathway[1].

IC50 & Target

Argininosuccinate synthetase (ASS1)[1]
In Vitro

LM2I (0µM~10µM, 48h) shows strong inhibitory effect in CRC cell lines[1].
LM2I (2µM, 15d) inhibits the EGFR pathway in colorectal cancer cells[1].
LM2I inhibits colorectal cancer cells via EGFR[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LM2I Related Antibodies

Cell Viability Assay[1]

Cell Line: CRC cell（HT29, SW480, SW620, HCT116, LoVo, RKO, and DLD1)
Concentration: 0µM,1.25µM,2.5µM,3.75µM,5.00µM,6.25µM,7.5µM,8.75µM,10µM
Incubation Time: 48h
Result: Inhibited the viability of HT29, SW480, SW620, HCT116, LoVo, RKO, and DLD1 cells.

Cell Proliferation Assay[1]

Cell Line: HT29 and SW480 cells
Concentration: 0.75µM,1µM
Incubation Time: 14d
Result: Had almost no effect on EGFR-KO cells.

Western Blot Analysis[1]

Cell Line: HT29, SW480, SW620, HCT116, LoVo, RKO, DLD1 cell
Concentration: 2µM
Incubation Time: 0~15d
Result: EGFR protein levels were higher than control group.Time-dependently inhibited the protein levels of EGFR and significantly reduced relative to phosphorylation.
In Vivo

LM2I (2.5 mg/kg/day, ip, every other day for 28 days) inhibits tumor growth in nude mice xenograft model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic BALB/c nude mice xenograft model(injected subcutaneously into the flank region with HT29 cell)[1].
Dosage: 2.5 mg/kg/day
Administration: Intraperitoneal injection,every other day for 28 days
Result: The tumor weight was significantly lower than that of the control group, and the tumor cell density was lower.
Molecular Weight

860.13

Formula

C47H77N3O11
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C[C@]2([H])[C@](C=C[C@@]3([H])[C@@]2([H])C[C@H](O[C@@H]4O[C@@H](C)[C@H](OC)[C@@H](OC)[C@H]4OC)C3)([H])[C@]1([H])C5)[C@H](C)[C@@H](O[C@@H]6O[C@H](C)[C@@H](N(CC(O)CN7CCNCC7)C)CC6)CCC[C@H](CC)OC5=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (116.26 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1626 mL 5.8131 mL 11.6261 mL
5 mM 0.2325 mL 1.1626 mL 2.3252 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1626 mL 5.8131 mL 11.6261 mL 29.0654 mL
5 mM 0.2325 mL 1.1626 mL 2.3252 mL 5.8131 mL
10 mM 0.1163 mL 0.5813 mL 1.1626 mL 2.9065 mL
15 mM 0.0775 mL 0.3875 mL 0.7751 mL 1.9377 mL
20 mM 0.0581 mL 0.2907 mL 0.5813 mL 1.4533 mL
25 mM 0.0465 mL 0.2325 mL 0.4650 mL 1.1626 mL
30 mM 0.0388 mL 0.1938 mL 0.3875 mL 0.9688 mL
40 mM 0.0291 mL 0.1453 mL 0.2907 mL 0.7266 mL
50 mM 0.0233 mL 0.1163 mL 0.2325 mL 0.5813 mL
60 mM 0.0194 mL 0.0969 mL 0.1938 mL 0.4844 mL
80 mM 0.0145 mL 0.0727 mL 0.1453 mL 0.3633 mL
100 mM 0.0116 mL 0.0581 mL 0.1163 mL 0.2907 mL
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LM2I Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LM2I2055494-50-1OthersSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesCRC cell linesBALB/c female miceColorectal cancer.Inhibitorinhibitorinhibit

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