2. Others Metabolic Enzyme/Protease
  3. Others Ser/Thr Protease
  4. LM2I

LM2I is a derivative of Spinosyn A (SPA). LM2I is argininosuccinate synthase (ASS1) enzyme activator, and tumor inhibitor that directly interact with ASS1. LM2I has significant antiproliferative activity in seven colorectal cancer cell-lines and xenograft tumors of colorectal cancer. LM2I inhibits colorectal cancer cell growth via the EGFR pathway.

For research use only. We do not sell to patients.

LM2I Chemical Structure

LM2I Chemical Structure

CAS No. : 2055494-50-1

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Based on 1 publication(s) in Google Scholar

LM2I Related Antibodies

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Description

LM2I is a derivative of Spinosyn A (SPA). LM2I is argininosuccinate synthase (ASS1) enzyme activator, and tumor inhibitor that directly interact with ASS1. LM2I has significant antiproliferative activity in seven colorectal cancer cell-lines and xenograft tumors of colorectal cancer. LM2I inhibits colorectal cancer cell growth via the EGFR pathway[1].

IC50 & Target

Argininosuccinate synthetase (ASS1)[1]
In Vitro

LM2I (0µM~10µM, 48h) shows strong inhibitory effect in CRC cell lines[1].
LM2I (2µM, 15d) inhibits the EGFR pathway in colorectal cancer cells[1].
LM2I inhibits colorectal cancer cells via EGFR[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LM2I Related Antibodies

Cell Viability Assay[1]

Cell Line: CRC cell（HT29, SW480, SW620, HCT116, LoVo, RKO, and DLD1)
Concentration: 0µM,1.25µM,2.5µM,3.75µM,5.00µM,6.25µM,7.5µM,8.75µM,10µM
Incubation Time: 48h
Result: Inhibited the viability of HT29, SW480, SW620, HCT116, LoVo, RKO, and DLD1 cells.

Cell Proliferation Assay[1]

Cell Line: HT29 and SW480 cells
Concentration: 0.75µM,1µM
Incubation Time: 14d
Result: Had almost no effect on EGFR-KO cells.

Western Blot Analysis[1]

Cell Line: HT29, SW480, SW620, HCT116, LoVo, RKO, DLD1 cell
Concentration: 2µM
Incubation Time: 0~15d
Result: EGFR protein levels were higher than control group.Time-dependently inhibited the protein levels of EGFR and significantly reduced relative to phosphorylation.
In Vivo

LM2I (2.5 mg/kg/day, ip, every other day for 28 days) inhibits tumor growth in nude mice xenograft model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic BALB/c nude mice xenograft model(injected subcutaneously into the flank region with HT29 cell)[1].
Dosage: 2.5 mg/kg/day
Administration: Intraperitoneal injection,every other day for 28 days
Result: The tumor weight was significantly lower than that of the control group, and the tumor cell density was lower.
Molecular Weight

860.13

Formula

C47H77N3O11
CAS No.
SMILES

O=C(C1=C[C@]2([H])[C@](C=C[C@@]3([H])[C@@]2([H])C[C@H](O[C@@H]4O[C@@H](C)[C@H](OC)[C@@H](OC)[C@H]4OC)C3)([H])[C@]1([H])C5)[C@H](C)[C@@H](O[C@@H]6O[C@H](C)[C@@H](N(CC(O)CN7CCNCC7)C)CC6)CCC[C@H](CC)OC5=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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LM2I Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LM2I2055494-50-1OthersSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesCRC cell linesBALB/c female miceColorectal cancer.Inhibitorinhibitorinhibit

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