Lucifer Yellow CH dipotassium salt

Name: Lucifer Yellow CH dipotassium salt
Brand: MedChemExpress
SKU: HY-W127715
Rating: 4.5 (1 reviews)

Cat. No.: HY-W127715 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Lucifer Yellow CH dipotassium is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.

For research use only. We do not sell to patients.

Lucifer Yellow CH dipotassium salt Chemical Structure

CAS No. : 71206-95-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 172	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 172	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Lucifer Yellow CH dipotassium salt:

Lucifer Yellow CH dilithium salt Get quote
Lucifer yellow CH ammonium Get quote

1 Publications Citing Use of MCE Lucifer Yellow CH dipotassium salt

•Free Radic Biol Med. 2021 Dec 6;178:271-294. [Abstract]

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

In Vitro

Preparation of Lucifer Yellow CH (dipotassium) working solution
1 Preparation of the stock solution
Dissolve Lucifer Yellow CH (dipotassium) in DDH₂O to obtain 1 mg/mL of Lucifer Yellow CH (dipotassium).
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Preparation of Lucifer Yellow CH (dipotassium) working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5% of Lucifer Yellow CH (dipotassium) working solution.
Note: Please adjust the concentration of Lucifer Yellow CH (dipotassium) working solution according to the actual situation.

Cell staining
1. Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2. Add 1 mL of Lucifer Yellow CH (dipotassium) working solution, and then incubate at room temperature for 5-30 minutes.
3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4. Wash twice with PBS, 5 minutes each time.
5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of Lucifer Yellow CH (dipotassium) working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

521.57

Formula

C₁₃H₉K₂N₅O₉S₂

CAS No.

71206-95-6

Appearance

Solid

Color

Yellow to orange

Emission (Em)

650

Excitation (Ex)

450

SMILES

O=C(NN1C(C2=CC(S(=O)(O[K])=O)=CC3=C(C(S(=O)(O[K])=O)=CC(C1=O)=C23)N)=O)NN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (159.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (9.59 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9173 mL	9.5864 mL	19.1729 mL
5 mM	0.3835 mL	1.9173 mL	3.8346 mL
10 mM	0.1917 mL	0.9586 mL	1.9173 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (283 KB) SDS (393 KB)

COA (261 KB) HNMR (287 KB) LCMS (272 KB)

Handling Instructions (2659 KB)

References

[1]. Klein M, et al. Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92. [Content Brief]

Cell Assay	MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Klein M, et al. Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.9173 mL	9.5864 mL	19.1729 mL	47.9322 mL
H₂O / DMSO	5 mM	0.3835 mL	1.9173 mL	3.8346 mL	9.5864 mL
DMSO	10 mM	0.1917 mL	0.9586 mL	1.9173 mL	4.7932 mL
	15 mM	0.1278 mL	0.6391 mL	1.2782 mL	3.1955 mL
	20 mM	0.0959 mL	0.4793 mL	0.9586 mL	2.3966 mL
	25 mM	0.0767 mL	0.3835 mL	0.7669 mL	1.9173 mL
	30 mM	0.0639 mL	0.3195 mL	0.6391 mL	1.5977 mL
	40 mM	0.0479 mL	0.2397 mL	0.4793 mL	1.1983 mL
	50 mM	0.0383 mL	0.1917 mL	0.3835 mL	0.9586 mL
	60 mM	0.0320 mL	0.1598 mL	0.3195 mL	0.7989 mL
	80 mM	0.0240 mL	0.1198 mL	0.2397 mL	0.5992 mL
	100 mM	0.0192 mL	0.0959 mL	0.1917 mL	0.4793 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.