Maprotiline

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Maprotiline is a highly selective noradrenergic reuptake inhibitor that has strong antidepressant, antitumor and neuropathic pain-relieving effects. Maprotiline induces cancer cell apoptosis by targeting the ERK signaling pathway and CRABP1.

For research use only. We do not sell to patients.

Maprotiline Chemical Structure

CAS No. : 10262-69-8

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Other In-stock Forms of Maprotiline:

Maprotiline hydrochloride

Other Forms of Maprotiline:

Maprotiline hydrochloride In-stock
Maprotiline (hydrochloride) (Standard) Get quote

2 Publications Citing Use of MCE Maprotiline

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
DG-75	EC₅₀	37.5 μM Compound: 1	Antiproliferative activity against human DG75 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human DG75 cells after 72 hrs by Alamar blue assay	[PMID: 24333581]
Ventricular myocyte	IC₅₀	31 μM Compound: Maprotiline	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]

In Vitro

Maprotiline (0-20 μM, 0-72 h) inhibits the growth of Huh7 and HepG2 cells and induces cell apoptosis^[1].
Maprotiline (0-20 μM, 0-72 h) inhibits the metastasis of liver cancer cells^[1].
Maprotiline (0-20 μM, 72 h) affects the ERK pathway and inhibits the phosphorylation of SREBP2 in HepG2 and Huh7 cells^[1].
Maprotiline targets CRABP1 and regulates cholesterol biosynthesis in HCC cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Invasion Assay^[1]

Cell Line:	The human HCC cell lines Huh7 and HepG2
Concentration:	0, 10, 20 μM
Incubation Time:	24 hours
Result:	Restrained HCC cells migration with inhibition of epithelial-mesenchymal transition (EMT).

Cell Viability Assay^[1]

Cell Line:	The human HCC cell lines Huh7 and HepG2
Concentration:	0, 10, 20 μM
Incubation Time:	0, 24, 48, 72, 96, 120 hours
Result:	Triggered cell apoptosis and inhibited the cell viability of Huh7 and HepG2 cells in a dose- and time-dependent manner.

Western Blot Analysis^[1]

Cell Line:	The human HCC cell lines Huh7 and HepG2
Concentration:	0, 10, 20 μM
Incubation Time:	72 hours
Result:	Inhibited cholesterol biosynthesis in HCC Cells.

In Vivo

Maprotiline (20-40 mg/kg, intraperitoneal injection, twice a week for three weeks) inhibits liver cancer tumor growth in mice^[1].
Maprotiline (3-30 mg/kg, intravenous injection, single dose) combined with the synthetic cannabinoid WIN 55,212-2 effectively reduces neuropathic pain in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nude mice (BALB/C nu/nu, 4–6 weeks old, female)^[1]
Dosage:	20, 40 mg/kg
Administration:	Intraperitoneal injection (i.p.); twice a week; 3 weeks
Result:	Decreased the cholesterol levels in serum and tumors and suppressed the growth of Huh7-derived tumor xenografts without obvious toxic effect.

Animal Model:	Male Balb-c mice (25–30 g)^[2]
Dosage:	3, 10, 30 mg/kg; single dose
Administration:	Intravenous injection (i.v.)
Result:	Attenuated pain-related behaviours in neuropathic mice.

Clinical Trial

Molecular Weight

277.40

Formula

C₂₀H₂₃N

CAS No.

10262-69-8

SMILES

CNCCCC12C3=C(C=CC=C3)C(CC2)C4=CC=CC=C14

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zheng C, et al. Maprotiline Suppresses Cholesterol Biosynthesis and Hepatocellular Carcinoma Progression Through Direct Targeting of CRABP1. Front Pharmacol. 2021 May 20. 12:689767. [Content Brief]

[2]. Gunduz O, et al. Analysis of the anti-allodynic effects of combination of a synthetic cannabinoid and a selective noradrenaline re-uptake inhibitor in nerve injury-induced neuropathic mice. Eur J Pain. 2016 Mar. 20(3):465-71.

Maprotiline Related Classifications

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Maprotiline10262-69-8AutophagyApoptosisERKExtracellular signal regulated kinaseshepatocellular carcinomacholesterol biosynthesisMAPK/ERK signaling pathwaySREBP2CRABP1antidepressantanticancerapoptosisautophagyInhibitorinhibitorinhibit

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