Maprotiline hydrochloride

Name: Maprotiline hydrochloride
Brand: MedChemExpress
SKU: HY-B0444
Rating: 5.0 (2 reviews)

Cat. No.: HY-B0444 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Maprotiline hydrochloride is a highly selective noradrenergic reuptake inhibitor that has strong antidepressant, antitumor and neuropathic pain-relieving effects with oral activity. Maprotiline hydrochloride induces cancer cell apoptosis by targeting the ERK signaling pathway and CRABP1.

For research use only. We do not sell to patients.

Maprotiline hydrochloride Chemical Structure

CAS No. : 10347-81-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Maprotiline hydrochloride:

Maprotiline-d₅ hydrochloride Get quote
Maprotiline-d₃ hydrochloride Get quote
Maprotiline Get quote
Maprotiline (hydrochloride) (Standard) Get quote

2 Publications Citing Use of MCE Maprotiline hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Maprotiline (0-20 μM, 0-72 h) hydrochloride inhibits the growth of Huh7 and HepG2 cells and induces cell apoptosis^[1].
Maprotiline (0-20 μM, 0-72 h) hydrochloride inhibits the metastasis of liver cancer cells^[1].
Maprotiline (0-20 μM, 72 h) hydrochloride affects the ERK pathway and inhibits the phosphorylation of SREBP2 in HepG2 and Huh7 cells^[1].
Maprotiline hydrochloride targets CRABP1 and regulates cholesterol biosynthesis in HCC cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Invasion Assay^[1]

Cell Line:	The human HCC cell lines Huh7 and HepG2
Concentration:	0, 10, 20 μM
Incubation Time:	24 h
Result:	Restrained HCC cells migration with inhibition of epithelial-mesenchymal transition (EMT).

Cell Viability Assay^[1]

Cell Line:	The human HCC cell lines Huh7 and HepG2
Concentration:	0, 10, 20 μM
Incubation Time:	0, 24, 48, 72, 96, 120 h
Result:	Triggered cell apoptosis and inhibited the cell viability of Huh7 and HepG2 cells in a dose- and time-dependent manner.

Western Blot Analysis^[1]

Cell Line:	The human HCC cell lines Huh7 and HepG2
Concentration:	0, 10, 20 μM
Incubation Time:	72 h
Result:	Inhibited cholesterol biosynthesis in HCC Cells.

In Vivo

Maprotiline (20-40 mg/kg, intraperitoneal injection, twice a week for three weeks) hydrochloride inhibits liver cancer tumor growth in mice^[1].
Maprotiline (3-30 mg/kg, intravenous injection, single dose) hydrochloride combined with the synthetic cannabinoid WIN 55,212-2 effectively reduces neuropathic pain in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nude mice (BALB/C nu/nu, 4–6 weeks old, female)^[1]
Dosage:	20, 40 mg/kg
Administration:	Intraperitoneal injection (i.p.); twice a week; 3 weeks
Result:	Decreased the cholesterol levels in serum and tumors and suppressed the growth of Huh7-derived tumor xenografts without obvious toxic effect.

Animal Model:	Male Balb-c mice (25–30 g)^[2]
Dosage:	3, 10, 30 mg/kg; single dose
Administration:	Intravenous injection (i.v.)
Result:	Attenuated pain-related behaviours in neuropathic mice.

Clinical Trial

Molecular Weight

313.86

Formula

C₂₀H₂₄ClN

CAS No.

10347-81-6

Appearance

Solid

Color

White to off-white

SMILES

CNCCCC1(CC2)C3=CC=CC=C3C2C4=CC=CC=C14.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (265.50 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.2 mg/mL (7.01 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1861 mL	15.9307 mL	31.8613 mL
5 mM	0.6372 mL	3.1861 mL	6.3723 mL
10 mM	0.3186 mL	1.5931 mL	3.1861 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2 mg/mL (6.37 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (282 KB) SDS (392 KB)

COA (253 KB) HNMR (257 KB) LCMS (250 KB)

Handling Instructions (2659 KB)

References

[1]. Zheng C, et al. Maprotiline Suppresses Cholesterol Biosynthesis and Hepatocellular Carcinoma Progression Through Direct Targeting of CRABP1. Front Pharmacol. 2021 May 20. 12:689767. [Content Brief]

[2]. Gunduz O, et al. Analysis of the anti-allodynic effects of combination of a synthetic cannabinoid and a selective noradrenaline re-uptake inhibitor in nerve injury-induced neuropathic mice. Eur J Pain. 2016 Mar. 20(3):465-71.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1861 mL	15.9307 mL	31.8613 mL	79.6534 mL
H₂O / DMSO	5 mM	0.6372 mL	3.1861 mL	6.3723 mL	15.9307 mL
DMSO	10 mM	0.3186 mL	1.5931 mL	3.1861 mL	7.9653 mL
	15 mM	0.2124 mL	1.0620 mL	2.1241 mL	5.3102 mL
	20 mM	0.1593 mL	0.7965 mL	1.5931 mL	3.9827 mL
	25 mM	0.1274 mL	0.6372 mL	1.2745 mL	3.1861 mL
	30 mM	0.1062 mL	0.5310 mL	1.0620 mL	2.6551 mL
	40 mM	0.0797 mL	0.3983 mL	0.7965 mL	1.9913 mL
	50 mM	0.0637 mL	0.3186 mL	0.6372 mL	1.5931 mL
	60 mM	0.0531 mL	0.2655 mL	0.5310 mL	1.3276 mL
	80 mM	0.0398 mL	0.1991 mL	0.3983 mL	0.9957 mL
	100 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7965 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.